IFO50428 CMK-86

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   IFO50428 Cell Name   CMK-86
Profile   This cell line expresses GPIIb/IIIa. Contains alpha-granule. Contains platelet peroxidase activity. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute megakaryoblastic leukemia Metastasis   
Tissue Metastasized    Genetics   from patient with Down's syndrome
Life Span   infinite Crisis PDL   
Morphology   myeloblast-like Character   leukemia, acute megakaryoblastic
Classify   tumor Established by   Sato, T. et al.
Registered by   Sato,T. Regulation for Distribution   
Comment   This cell line is useful for studying megakaryocytopoiesis. Year   1994
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2804
Reference
Pubmed id:2665839Growth and differentiation of a human megakaryoblastic cell line, CMK.
Komatsu N,Suda T,Moroi M,Tokuyama N,Sakata Y,Okada M,Nishida T,Hirai Y,Sato T,Fuse A
Blood. 1989 Jul;74(1):42-8
Pubmed id:2527057Establishment of a human leukaemic cell line (CMK) with megakaryocytic characteristics from a Down's syndrome patient with acute megakaryoblastic leukaemia.
Sato T,Fuse A,Eguchi M,Hayashi Y,Ryo R,Adachi M,Kishimoto Y,Teramura M,Mizoguchi H,Shima Y
Br J Haematol. 1989 Jun;72(2):184-90
Pubmed id:2955817The c-sis gene expression in cells from a patient with acute megakaryoblastic leukemia and Down's syndrome.
Sunami S,Fuse A,Simizu B,Eguchi M,Hayashi Y,Sugita K,Nakazawa S,Okimoto Y,Sato T,Nakajima H
Blood. 1987 Aug;70(2):368-71
Pubmed id:3035541Molecular cloning of cDNAs of human liver and placenta NADH-cytochrome b5 reductase.
Yubisui T,Naitoh Y,Zenno S,Tamura M,Takeshita M,Sakaki Y
Proc Natl Acad Sci U S A. 1987 Jun;84(11):3609-13

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LOT Information

Cell No.   IFO50428 Cell Name   
LOT No.   02142007 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS lot GIBCO 484375) Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   Dilution. (Small portion of cells becomes adherent in culture without passage.)
Doubling Time   68 hrs Cell Number in Vial (cells/1ml)   8.0 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (>4 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:11
D7S820:11,12
D16S539:9,11
VWA:18
TH01:7
AM:X,Y
TPOX:9,11
CSF1PO:11,13
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50428 Cell Name   CMK-86
LOT No.   977 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   Unknown (>3 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50428 Cell Name   CMK-86
LOT No.   07282017 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1.5 x 105 cells/mL Methods for Passages   Simple dilution (suspension culture). The growth at the initiation of culture was slow.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   7.6 x 10^5
Viability at cell freezing (%)   86.9 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   86.9 Additional information   
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