IFO50495 Neuro-2aTG
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50495 | Cell Name | Neuro-2aTG |
|---|---|---|---|
| Profile | This cell line is HPRT-deficient clone from EMS-mutagnenized Neuro-2a cells. HAT-sensitive, 6-thioguanine-registant. | Other Name | |
| Animal | mouse | Strain | |
| Genus | Mus | Species | musclus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | neural | |
| Case history | neuroblastoma | Metastasis | |
| Tissue Metastasized | Genetics | HPRT-deficient | |
| Life Span | infinite | Crisis PDL | |
| Morphology | neuron-like | Character | neuroblastoma, HPRT-deficient Neuro-2a cell line |
| Classify | mutant | Established by | Satoh, M. and Takeuchi, M. |
| Registered by | Satoh,M. | Regulation for Distribution | |
| Comment | Year | 1998 | |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum. For selection, add 5 ug/ml 6-thioguanine. | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | region of spinal cord |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
LOT Information
| Cell No. | IFO50495 | Cell Name | Neuro-2aTG |
|---|---|---|---|
| LOT No. | 1088 | Lot Specification | distribution |
| Medium | D.MEM, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | 22 hr | Cell Number in Vial (cells/1ml) | 7 x 10^5 |
| Viability at cell freezing (%) | 87 | Antibiotics Used | free |
| Passage Number | P10 after subcloning | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, MD, AST |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information | Lot No. is labeled as R953F1088. |