IFO50496 PC-12TG
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | IFO50496 | Cell Name | PC-12TG |
|---|---|---|---|
| Profile | HAT-sensitive, 6-thioguanine-resistant. | Other Name | |
| Animal | rat | Strain | |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | neural | |
| Case history | pheochromocytoma | Metastasis | |
| Tissue Metastasized | Genetics | HPRT-deficient | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-polygonal | Character | pheochromocytoma, HPRT-deficient PC-12 cell line |
| Classify | mutant | Established by | Satoh, M. and Takeuchi, M. |
| Registered by | Satoh,M. | Regulation for Distribution | |
| Comment | Year | 1998 | |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum. For selection, add 5 ug/ml 6-thioguanine. | Methods for Passages | trypsin-EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | adrenal medulla |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
LOT Information
| Cell No. | IFO50496 | Cell Name | PC-12TG |
|---|---|---|---|
| LOT No. | 1090 | Lot Specification | distribution |
| Medium | D.MEM, 90% ; FBS, 10% | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | trypsin-EDTA | |
| Doubling Time | 38 hr | Cell Number in Vial (cells/1ml) | 2 x 10^6 |
| Viability at cell freezing (%) | 94 | Antibiotics Used | free |
| Passage Number | P12 after subcloning | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% serum (final conc.) - culture medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |