JCRB0005 FRSK

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0005 Cell Name   FRSK
Profile   Fetal rat skin keratinocyte cell line. Other Name   
Animal   rat Strain   inbred SD
Genus   Rattus Species   norvegicus
Sex    Age   
Identity   not done Tissue for Primary Cancer   skin
Case history   normal Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   epidermal cells
Classify   transformed Established by   Indo,K. and Wilson,R.B.
Registered by   Kuroki,T. Regulation for Distribution   
Comment   Doubling time = 15 hrs. Year   1985
Medium   Eagle's minimal essential medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   skin keratinocyte

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5501
Reference
Pubmed id:2428814Down regulation of phorbol diester receptors by proteolytic degradation of protein kinase C in a cultured cell line of fetal rat skin keratinocytes.
Chida K,Kato N,Kuroki T
J Biol Chem. 1986 Oct 5;261(28):13013-8
Pubmed id:6168513Effect of tumor promote phorbol esters on the in vitro keratinization of rat epidermal cells in normal, premalignant and malignant stages.
Indo K,Miyaji H
Gan. 1981 Feb;72(1):123-31
Pubmed id:480376Qualitative changes in the biologic characteristics of cultured fetal rat keratinizing epidermal cells during the process of malignant transformation after benzo[a]pyrene treatment.
Indo K,Miyaji H
J Natl Cancer Inst. 1979 Oct;63(4):1017-27
Pubmed id:218222Altered pattern of growth and differentiation in human keratinocytes infected by simian virus 40.
Steinberg ML,Defendi V
Proc Natl Acad Sci U S A. 1979 Feb;76(2):801-5
Pubmed id:894748Fetal rat keratinizing epidermal cells in culture: effects of long-term treatment by benzo[a]pyrene on their growth characteristics.
Indo K,Wilson RB
J Natl Cancer Inst. 1977 Sep;59(3):867-80
Pubmed id:302145Terminal differentiation of cultured human epidermal cells.
Green H
Cell. 1977 Jun;11(2):405-16

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LOT Information

Cell No.   JCRB0005 Cell Name   
LOT No.   04182007 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   0.5-1.1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA at 37C for 5-10 min.
Doubling Time   30-40 hrs (avarage) Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   P51 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS -EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0005 Cell Name   FRSK
LOT No.   021593 Lot Specification   distribution
Medium   Ham's F12 medium with 10% fetal bovine serum (Hyclone 115995) Temperature   37 C
Cell Density at Seeding   1.22x10^5 cells/ml Methods for Passages   Wash once with PBS, treated with 0.02% EDTA and 0.25% trypsin at 37 C for 15 minutes.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   P52 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0005 Cell Name   FRSK
LOT No.   071190 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   1/10 Methods for Passages   0.02 % EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   75.0 Antibiotics Used   free
Passage Number   P50 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0005 Cell Name   FRSK
LOT No.   021786 Lot Specification   distribution
Medium   MEM + FCS 10% Temperature   37
Cell Density at Seeding   1/10 Methods for Passages   0.02 % EDTA and 0.25% Trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P49 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0005 Cell Name   FRSK
LOT No.   05082015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   4.7 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P50 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   91 Additional information   
Cell No.   JCRB0005 Cell Name   FRSK
LOT No.   03102022 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   approx 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day (log phase) Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   P50 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
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