JCRB0056 LU65C

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0056 Cell Name   LU65C
Profile   giant cell carcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   64
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung cancer Metastasis   
Tissue Metastasized    Genetics   lung, carcinoma
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   tumor Established by   Suzuki,K. et al.
Registered by   Suzuki,K. Regulation for Distribution   Permission for the distribution required.
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   giant cell carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3014
Reference
Pubmed id:2112525A simple, general method for detecting retroviral RNAs expressed in cells.
Harada F,Hirose Y,Tanaka M,Sasaki T,Kamiya H,Miura K
Jpn J Cancer Res. 1990 Mar;81(3):232-7
Pubmed id:2659722Purification and partial primary sequence of a chemotactic protein for polymorphonuclear leukocytes derived from human lung giant cell carcinoma LU65C cells.
Suzuki K,Miyasaka H,Ota H,Yamakawa Y,Tagawa M,Kuramoto A,Mizuno S
J Exp Med. 1989 Jun 1;169(6):1895-901
Pubmed id:3935627Giant cell carcinomas of the lung producing colony-stimulating factor in vitro and in vivo.
Yamada T,Hirohashi S,Shimosato Y,Kodama T,Hayashi S,Ogura T,Gamou S,Shimizu N
Jpn J Cancer Res. 1985 Oct;76(10):967-76

Images



LOT Information

Cell No.   JCRB0056 Cell Name   LU65C
LOT No.   052590 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   dilution 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P16 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0056 Cell Name   LU65C
LOT No.   111185 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:10
D7S820:11,12
D16S539:9,11
VWA:18
TH01:6
AM:X
TPOX:8,11
CSF1PO:13
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0056 Cell Name   LU65C
LOT No.   02122016 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.9 - 1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Some non-adherent cells were also collected, and used for passage.
Doubling Time   approx. 34 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   88 Additional information   
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