JCRB0057 LU99B

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0057 Cell Name   LU99B
Profile   Lung giant cell carcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   63
Identity   available Tissue for Primary Cancer   lung (cancer)
Case history   lung cancer Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   tumor Established by   Suzuki,K. et al.
Registered by   Suzuki,K. Regulation for Distribution   Permission for the distribution required.
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Mechanical dispersion.
Cell Number on Passage   split ratio=1/10 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   giant cell carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2801
Reference
Pubmed id:3935627Giant cell carcinomas of the lung producing colony-stimulating factor in vitro and in vivo.
Yamada T,Hirohashi S,Shimosato Y,Kodama T,Hayashi S,Ogura T,Gamou S,Shimizu N
Jpn J Cancer Res. 1985 Oct;76(10):967-76

Images



LOT Information

Cell No.   JCRB0057 Cell Name   LU99B
LOT No.   100296 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0057 Cell Name   LU99B
LOT No.   062294 Lot Specification   Distribution
Medium   RMPI1640 medium with 10% heat inactivated fetal bovine serum(JR Scientific CO19407) Temperature   37 C
Cell Density at Seeding   1.0x10^4 cells/ml Methods for Passages   Mechanical dispersion by loose pippeting.
Doubling Time   18.2 hrs Cell Number in Vial (cells/1ml)   1.14x10^6
Viability at cell freezing (%)   92.5 Antibiotics Used   free
Passage Number   P15 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:10,11
D7S820:11
D16S539:9
VWA:18
TH01:9
AM:X
TPOX:11
CSF1PO:10,15
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cult.medium+5%DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0057 Cell Name   LU99B
LOT No.   102885 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   Mechanical dispersion.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2x10^6
Viability at cell freezing (%)   95% Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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