JCRB1054 Hep G2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1054 Cell Name   Hep G2
Profile   Hepatoblastoma-derived (previously thought to be hepatocellular carcinoma-derived) cell line. Other Name   HepG2
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   15
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   liver cancer Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   Hepatoblastoma cell line. alpha-fetoprotein (+), HLA-A 02/2402.
Classify   tumor Established by   Knowless,B.B.
Registered by    Regulation for Distribution   
Comment   Mycoplasmas were not detected three months after the decontamination. Year   2003
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. or GIT medium. Methods for Passages   Cells harvested after treatment with 0.25% trypsin.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   differentiated liver carcinoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0027
Reference
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J Biol Chem. 1997 Jun 6;272(23):14954-60
Pubmed id:8985401Formation of native hepatitis C virus glycoprotein complexes.
Deleersnyder V,Pillez A,Wychowski C,Blight K,Xu J,Hahn YS,Rice CM,Dubuisson J
J Virol. 1997 Jan;71(1):697-704
Pubmed id:8971024Recombinant adenoviruses with large deletions generated by Cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo.
Lieber A,He CY,Kirillova I,Kay MA
J Virol. 1996 Dec;70(12):8944-60
Pubmed id:8943055Biochemical characterization and intracellular localization of the Menkes disease protein.
Yamaguchi Y,Heiny ME,Suzuki M,Gitlin JD
Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):14030-5
Pubmed id:8798649Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene.
Jang SI,Steinert PM,Markova NG
J Biol Chem. 1996 Sep 27;271(39):24105-14
Pubmed id:8709260Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells.
Goodrum FD,Shenk T,Ornelles DA
J Virol. 1996 Sep;70(9):6323-35
Pubmed id:8764004Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases.
Benn J,Su F,Doria M,Schneider RJ
J Virol. 1996 Aug;70(8):4978-85
Pubmed id:8706128Alpha L beta 2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1, a cytoplasmic regulatory molecule.
Kolanus W,Nagel W,Schiller B,Zeitlmann L,Godar S,Stockinger H,Seed B
Cell. 1996 Jul 26;86(2):233-42
Pubmed id:8622980Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts.
Lewis W,Levine ES,Griniuviene B,Tankersley KO,Colacino JM,Sommadossi JP,Watanabe KA,Perrino FW
Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3592-7
Pubmed id:8626493Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms.
Klemm DJ,Colton LA,Ryan S,Routes JM
J Biol Chem. 1996 Apr 5;271(14):8082-8
Pubmed id:8626491The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells.
Roesler WJ,Crosson SM,Vinson C,McFie PJ
J Biol Chem. 1996 Apr 5;271(14):8068-74
Pubmed id:8626456Cellular internalization and degradation of antithrombin III-thrombin, heparin cofactor II-thrombin, and alpha 1-antitrypsin-trypsin complexes is mediated by the low density lipoprotein receptor-related protein.
Kounnas MZ,Church FC,Argraves WS,Strickland DK
J Biol Chem. 1996 Mar 15;271(11):6523-9
Pubmed id:8626812The proximal promoter of the human transglutaminase 3 gene. Stratified squamous epithelial-specific expression in cultured cells is mediated by binding of Sp1 and ets transcription factors to a proximal promoter element.
Lee JH,Jang SI,Yang JM,Markova NG,Steinert PM
J Biol Chem. 1996 Feb 23;271(8):4561-8
Pubmed id:8551615Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin.
Dubuisson J,Rice CM
J Virol. 1996 Feb;70(2):778-86
Pubmed id:8380141Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines.
Schardt C,Rotsch M,Erbil C,Göke R,Richter G,Havemann K
Exp Cell Res. 1993 Jan;204(1):22-9
Pubmed id:2176219Differential regulation of hepatic triglyceride lipase and 3-hydroxy-3-methylglutaryl-CoA reductase gene expression in a human hepatoma cell line, HepG2.
Busch SJ,Barnhart RL,Martin GA,Flanagan MA,Jackson RL
J Biol Chem. 1990 Dec 25;265(36):22474-9
Pubmed id:3034851Growth and hepatospecific gene expression of human hepatoma cells in a defined medium.
Darlington GJ,Kelly JH,Buffone GJ
In Vitro Cell Dev Biol. 1987 May;23(5):349-54
Pubmed id:6248960Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.
Knowles BB,Howe CC,Aden DP
Science. 1980 Jul 25;209(4455):497-9
Pubmed id:233137Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line.
Aden DP,Fogel A,Plotkin S,Damjanov I,Knowles BB
Nature. 1979 Dec 6;282(5739):615-6


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LOT Information

Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   04072011 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1g/L glucose) with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   07202010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.95-5.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.22x10^6
Viability at cell freezing (%)   94.1 Antibiotics Used   free
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,13
D7S820:10
D16S539:12,13
VWA:17
TH01:9
AM:X,Y
TPOX:8,9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)   86.89 Additional information   
Images
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   11042009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   0.9 - 1.8 x 10^5 cells/ml, passage twice a week. Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   88.3 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1054 Cell Name   
LOT No.   02272009 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   97.3 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS -DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1054 Cell Name   
LOT No.   07112007 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/ml (passage twice a week) Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   21 hrs Cell Number in Vial (cells/1ml)   3.9 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1054 Cell Name   
LOT No.   05152006 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.6 - 2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   11182004 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; GIBCO3762782S) Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 22 hrs Cell Number in Vial (cells/1ml)   5.6 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P12 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   08082003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   2-3 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.25% typsin. Subculture once a 4-6 days and medium change every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   98.3 Antibiotics Used   free
Passage Number   P10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   07302003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   2-4 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.25% trypsin for 10 min. at room temperature. Subculture every 4-6 days and medium change every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.1 x 10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   P9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   05272013 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (1 g/L glucose) with 10% fetal bovine serum. (FBS: GIBCO Cat. #10099) Temperature   37 C
Cell Density at Seeding   1.4 x 10^5 cells/mL Methods for Passages   Cells harvested after treatment with 0.25% trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   1.2 x 10^7
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   97.4 Additional information   
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   04202017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   90.1 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.1 Additional information   
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   07052018 Lot Specification   distribution
Medium   Dubecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.8 - 3.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Pssage twice a week.
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   94.6 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   83.4 Additional information   
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   08262020 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   04012022 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.3 - 2.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 day Cell Number in Vial (cells/1ml)   2.4 x 10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   96.0 Additional information   
Cell No.   JCRB1054 Cell Name   Hep G2
LOT No.   20240206 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (Nacalai Tesque #08156-65 lot L3G3526) with 10% heat-inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   2-4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA. (Nacalai Tesque #335554-64 lot L3K4072)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.84x10^6
Viability at cell freezing (%)   98.3 Antibiotics Used   free
Passage Number   p12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,13
D7S820:10
D16S539:12,13
VWA:17
TH01:9
AM:X,Y
TPOX:8,9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc.,CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   96.7 Additional information   
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