JCRB0220 NTI-4
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0220 | Cell Name | NTI-4 |
|---|---|---|---|
| Profile | Normal human fibroblast. | Other Name | NTI-4 |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | 9-week fetus | |
| Identity | available | Tissue for Primary Cancer | embryo |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | fetus, half body, bottom/fibroblast | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | fetus, half body, bottom/fibroblast |
| Classify | Established by | Inui,N. | |
| Registered by | Egashira,Y. | Regulation for Distribution | |
| Comment | Year | 1986 | |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum | Methods for Passages | Cells are treated with 0.25% trypsin. |
| Cell Number on Passage | 2x10^4 cells/ml | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | fetus body |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Images |
|---|
         |
LOT Information
| Cell No. | JCRB0220 | Cell Name | |
|---|---|---|---|
| LOT No. | 01112008 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS Lot; GIBCO 484375) | Temperature | 37 C |
| Cell Density at Seeding | 4 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | approx. 20 hrs | Cell Number in Vial (cells/1ml) | 7 x 10^5 |
| Viability at cell freezing (%) | 83 | Antibiotics Used | free |
| Passage Number | P18 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), LD |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0220 | Cell Name | |
|---|---|---|---|
| LOT No. | 042592 | Lot Specification | distribution |
| Medium | DMDM : 10% FCS | Temperature | 37 |
| Cell Density at Seeding | Methods for Passages | 0.25% trypsin | |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | ||
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |