JCRB0144.1D LYM-1.P3D

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0144.1D Cell Name   LYM-1.P3D
Profile   Serum free FAR-2.F60 rat cell culture from lymph node near colon cancer. Other Name   
Animal   rat Strain   JAR-2
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   lymph node
Case history   tissue obtained from lymph node near colon cancer of the JAR-2 rat given MNU(0.4mg/ml). The MNU was applied to a bowel directly of two-week-old rat and tumors were found three mo. later. The lymph node was cultured by roller tubes with DM170 and 2% FBS. Metastasis   
Tissue Metastasized    Genetics   Subclone of the LYM-1.P3. Morphologys and karyotypes were different from the LYM-1.P3B. Karyotype is lower diploid.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Culture fruid of the LYM-1 strongly inhibited a growth of AH-7974 and other several cell lines. The inhibition was strongly suppressed by heating.
Classify   serum free Established by   Kurayama,H. and Takaoka,T.
Registered by   Takaoka,T. Regulation for Distribution   free
Comment    Year   2004
Medium   DM201 medium with serum free condition. Methods for Passages   Cells harvested by gentle scraping.
Cell Number on Passage   1.3 x 10^4 cells/sq.cm. Race   
CO2 Conc.    Tissue Sampling   lymph node near colon cancer
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_U215
Reference
Pubmed id:9511820The value of LYM-1 cells for examining vacuole formation and loss of cell viability induced by culture supernates of Helicobacter pylori.
Wang XM,Kojima T,Satoh K,Taniguchi Y,Tokumaru K,Saifuku K,Seki M,Kihira K,Ido K,Uchida JY,Ohmori C,Takaoka T,Kimura K
J Med Microbiol. 1997 Aug;46(8):705-9

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LOT Information

Cell No.   JCRB0144.1D Cell Name   LYM-1.P3D
LOT No.   07152004 Lot Specification   distribution
Medium   DM201 serum free medium. Temperature   37 C
Cell Density at Seeding   2.0 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with scraper.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   91.0 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH,NP,G6PD examined. Rat proved.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   1 to 1 mixture of the culture medium and the FM-1 medium containing 10% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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