JCRB0245 RMT-1 M2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0245 Cell Name   RMT-1 M2
Profile   Rat mammary carcinoma cell line from rat mammary gland induced by single shot DMBA(20mg) administrated by oral. Other Name   
Animal   rat Strain   SD
Genus   Rattus Species   norvegicus
Sex   F Age   8 weeks
Identity    Tissue for Primary Cancer   mammary
Case history   single 20 mg DMBA(CAS 57-97-6) was administrated through oral. Twelve weeks later a 15 x 25-mm mammary tumor appeared in the right mammry gland. Metastasis   
Tissue Metastasized    Genetics   rat mammary myoepithelial tumor cells.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cells were prepared from the tumor.
Classify   tumor Established by   Hiragun,A.
Registered by   Hiragun,A. Regulation for Distribution   Not applied.
Comment    Year   1988
Medium   45% Ham's F12 medium, 45% Dulbecco's modified Eagle's medium and 10% fetal calf serum, supplemented with 10mM Hepes, 2x10^-8 M Na-Selenite, 1mM EGTA. Methods for Passages   Cells are treated with 0.05 % trypsin and 0.02 % EDTA
Cell Number on Passage   10^4-10^5 cells/35mm dish Race   
CO2 Conc.   5 % Tissue Sampling   mammary gland
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5361
Reference
Pubmed id:2411993Isolation of two syngeneic cell lines from a rat mammary carcinoma: growth factor production by neoplastic epithelial cells.
Hiragun A,Yoshida Y,Sato M,Tominaga T,Mitsui H
J Natl Cancer Inst. 1985 Sep;75(3):471-82

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LOT Information

Cell No.   JCRB0245 Cell Name   
LOT No.   020789 Lot Specification   distribution
Medium   HamF12:DMEM (see comment for details) Temperature   37
Cell Density at Seeding    Methods for Passages   0.05 % trypsin + 0.02 % EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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