JCRB0256 RCM-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0256 Cell Name   RCM-1
Profile   adenocarcinoma, well differentiated Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   rectum
Case history    Metastasis   
Tissue Metastasized    Genetics   rectum, adenocarcinoma, well differentiated
Life Span    Crisis PDL   
Morphology    Character   rectum, adenocarcinoma, well differentiated
Classify    Established by   Kataoka,H.
Registered by   Kataoka, H. Regulation for Distribution   Please send a reprint to Kataoka, H.
Comment   73 year-old, female. Partially mucinous. Secrete proteinase inhibitors, and CEA etc. Year   1989
Medium   45% RPMI1640 medium with 45% Ham's F12 medium and 10% fetal calf serum Methods for Passages   0.02% EDTA and 0.125% trypsin. Subculture after observation of dome formation. Dome is induced
Cell Number on Passage   split ratio=1/4,every 2-3 wks Race   
CO2 Conc.   5 % Tissue Sampling   rectum
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1648
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909263
Reference
Pubmed id:3198327A new long-term in vitro invasion assay using fibrous connective tissue matrices maintaining architectural characteristics of connective tissue.
Nabeshima K,Kataoka H,Koita H,Murayama T,Koono M
Invasion Metastasis. 1988;8(5):301-16

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LOT Information

Cell No.   JCRB0256 Cell Name   
LOT No.   09072005 Lot Specification   distribution
Medium   45% RPMI1640 medium and 45% Ham's F12 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   2 - 7 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 70 hrs Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   97.7 Antibiotics Used   free
Passage Number   P69 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0256 Cell Name   
LOT No.   091593 Lot Specification   distribution
Medium   RPMI 1640 + Ham's F12 (1:1) + FCS 10% Temperature   37
Cell Density at Seeding   1/4 per 2-3 weeks Methods for Passages   0.02% EDTA and 0.125% trypsin. Subculture after observation of dome formation. Dome is induced
Doubling Time    Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P65 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST, G6PD, LD, MD, NP, PEPB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0256 Cell Name   RCM-1
LOT No.   04252014 Lot Specification   distribution
Medium   1:1 mixture of RPMI1640 medium and Ham's F12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.4 - 1.9 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   3.7 x 10^6
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P68 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:10,12
D7S820:11,12
D16S539:11,12
VWA:20
TH01:9
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640/Ham's F12 CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
Cell No.   JCRB0256 Cell Name   RCM-1
LOT No.   01282021 Lot Specification   distribution
Medium   1:1 mixture of RPMI1640 medium and Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.2 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.125% trypsin.
Doubling Time   approx. 60 hrs. Cell Number in Vial (cells/1ml)   4.3 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P68 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640/F-12 CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   
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