JCRB9034 LLC-RK1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9034 Cell Name   LLC-RK1
Profile   an aneuploid cell line, (New Zealand white rabbit) Other Name   LLC-RK1
Animal   rabbit Strain   New Zealand white
Genus   Oryctolagus Species   cuniculus
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history   pooled normal kidneys from several rabbits Metastasis   No
Tissue Metastasized    Genetics   an aneuploid cell line
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   susceptible to viruses,leuvella,vaccinia & herpes.
Classify   transformed Established by   Hull,R.N. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepare from CCL 106. The horse serum requires careful selection as to quality. Year   1986
Medium   Medium 199 + horse serum 10% Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2579
Reference
Pubmed id:14277663DEVELOPMENT AND CHARACTERISTICS OF THE RABBIT KIDNEY CELL STRAIN, LLC-RK.
HULL RN,DWYER AC,CHERRY WR,TRITCH OJ
Proc Soc Exp Biol Med. 1965 Apr;118():1054-9




LOT Information

Cell No.   JCRB9034 Cell Name   
LOT No.   12192001 Lot Specification   distribution
Medium   Medium 199 with 10% horse serum Temperature   37 C
Cell Density at Seeding   1-2 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   55-60 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P244 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rabbit by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% HS-Medium 199 CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9034 Cell Name   LLC-RK1
LOT No.   100886 Lot Specification   distribution
Medium   Medium 199 with NaHCO3 1.12g/L and 10% heat inactivated horse serum Temperature   37
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P242 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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