JCRB9049 CV-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9049 Cell Name   CV-1
Profile   kidney, highly susceptible to SV40 infection Other Name   
Animal   monkey, African green Strain   
Genus   Cercopithecus Species   aethiops
Sex   M Age   adult
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   kidney
Life Span    Crisis PDL   
Morphology    Character   kidney, highly susceptible to SV40 infection
Classify    Established by   Jensen, F.C. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 70. Year   1986
Medium   MEM + FCS 10% (Current medium for propagation; Eagle's MEM with non-essential amino acids and 10% FBS) Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/3 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0229
Reference
Pubmed id:2546481Inhibition of herpes simplex virus infection by pine cone antitumor substances.
Fukuchi K,Sakagami H,Ikeda M,Kawazoe Y,Oh-Hara T,Konno K,Ichikawa S,Hata N,Kondo H,Nonoyama M
Anticancer Res. 1989 Mar-Apr;9(2):313-7
Pubmed id:2552915Inhibition of herpes simplex virus infection by tannins and related compounds.
Fukuchi K,Sakagami H,Okuda T,Hatano T,Tanuma S,Kitajima K,Inoue Y,Inoue S,Ichikawa S,Nonoyama M
Antiviral Res. 1989 Jun-Jul;11(5-6):285-97
Pubmed id:2851895Herpes simplex virus type 1 alpha gene containing plasmids can inhibit expression regulated from an alpha promoter in CV-1 but not HeLa cells.
Block T,Jordan R
Virus Res. 1988 Nov;11(4):269-79
Pubmed id:3869955Recombination and deletion of sequences in shuttle vector plasmids in mammalian cells.
Chakrabarti S,Joffe S,Seidman MM
Mol Cell Biol. 1985 Sep;5(9):2265-71
Pubmed id:6281760Efficient expression of Escherichia coli galactokinase gene in mammalian cells.
Schümperli D,Howard BH,Rosenberg M
Proc Natl Acad Sci U S A. 1982 Jan;79(2):257-61
Pubmed id:4285109Enhanced thymidine kinase activity following infection of green monkey kidney cells by simian adenoviruses, simian papovavirus SV40, and an adenovirus-SV40 "hybrid".
Kit S,Dubbs DR,DeTorres RA,Melnick JL
Virology. 1965 Nov;27(3):453-7
Pubmed id:14192657INFECTION OF HUMAN AND SIMIAN TISSUE CULTURES WITH ROUS SARCOMA VIRUS.
JENSEN FC,GIRARDI AJ,GILDEN RV,KOPROWSKI H
Proc Natl Acad Sci U S A. 1964 Jul;52():53-9

Images



LOT Information

Cell No.   JCRB9049 Cell Name   CV-1
LOT No.   10052007 Lot Specification   distribution
Medium   Eagle's minimam essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   3-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Split ratio = 1/2 - 1/4
Doubling Time   approx. 34 hrs Cell Number in Vial (cells/1ml)   5.5 x 10^5
Viability at cell freezing (%)   92.3 Antibiotics Used   free
Passage Number   P41 (ampoule label=09062007) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as African green monkey by NP, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   Ampules are mislabeled as Lot No.09062007.
Cell No.   JCRB9049 Cell Name   
LOT No.   02252002 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   5.0 x 10^5
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P41 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as African green monkey by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-base medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9049 Cell Name   CV-1
LOT No.   060895 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum and non-essential amino acids Temperature   37 C
Cell Density at Seeding   1.6x10^4 cells/sq.cm Methods for Passages   Cells are washed once with PBS(-) and treated with 0.05% trypsin and 0.02% EDTA at 37 C for 5 min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   82.4 Antibiotics Used   free
Passage Number   P42 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LDH,NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9049 Cell Name   CV-1
LOT No.   070892 Lot Specification   distribution
Medium   E.MEM with non essential amino acids (lot.61692) + 10% FCS (lot.J.R.S. DO10400) Temperature   37 C
Cell Density at Seeding   1.4x10^6 Methods for Passages   Wash once with PBS and treated with 0.02% EDTA and0.05% trypsin at 37 C for 5min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.08x10^6
Viability at cell freezing (%)   94.7 Antibiotics Used   free
Passage Number   P37 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LD,G6PD
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9049 Cell Name   CV-1
LOT No.   082990 Lot Specification   distribution
Medium   MEM + NEAA + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/3 Methods for Passages   0.01% or 0.02% EDTA and 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.5x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P33 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9049 Cell Name   CV-1
LOT No.   031887 Lot Specification   distribution
Medium   MEM + NEAA + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/3 Methods for Passages   0.01% or 0.02% EDTA and 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   6x10^5
Viability at cell freezing (%)   93% Antibiotics Used   free
Passage Number   P31 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9049 Cell Name   CV-1
LOT No.   11152016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.4 - 2.0 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   9.0 x 10^5
Viability at cell freezing (%)   90 Antibiotics Used   free
Passage Number   P40 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
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