JCRB1322 m5S
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1322 | Cell Name | m5S |
|---|---|---|---|
| Profile | Near-diproid mouse embryonic cell line. | Other Name | |
| Animal | mouse | Strain | ICR |
| Genus | Mus | Species | musclus |
| Sex | M | Age | 17.5-day p.c. |
| Identity | not done | Tissue for Primary Cancer | skin |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | Near-diproid chromosome number, contact inhibition, non-tumorigenic. Karyotype : 42,X,+15,+der(6)t(6;13)(D;B),+der(8)t(8;8)(E2;A2) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | normal |
| Classify | normal | Established by | Sasaki, M. S. |
| Registered by | Sasaki, M. S. | Regulation for Distribution | |
| Comment | Year | 2008 | |
| Medium | alpha-MEM with 10% FCS, HEPES(20mM), penicillin(100U/ml), Streptomycin(100ug/ml). | Methods for Passages | 0.25% trypsin and 0.02% EDTA in PBS(-) |
| Cell Number on Passage | Sprit ratio of about 1/10 | Race | |
| CO2 Conc. | 5% | Tissue Sampling | Skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:16819136 | Development, beam characterization and chromosomal effectiveness of X-rays of RBC characteristic X-ray generator. Endo S,Hoshi M,Takada J,Takatsuji T,Ejima Y,Saigusa S,Tachibana A,Sasaki MS J Radiat Res. 2006 Jun;47(2):103-12 |
| Pubmed id:1483264 | Changes of DNA methylation in protooncogenes in the process of radiation-induced transformation of mouse m5S/1M cells in vitro. Yasuzawa K,Kodama S,Kato M,Uehara Y,Tawa R,Hirose S,Sasaki MS,Okada S,Ono T Cancer Lett. 1992 Dec 24;67(2-3):157-66 |
| Pubmed id:1351528 | Induction of chromosome aberrations by monochromatic X-rays with resonance energy of phosphorus K-shell absorption edge. Saigusa S,Ejima Y,Kobayashi K,Sasaki MS Int J Radiat Biol. 1992 Jun;61(6):785-90 |
| Pubmed id:1757472 | Neural cell adhesion molecule mediates contact-dependent inhibition of growth of near-diploid mouse fibroblast cell line m5S/1M. Aoki J,Umeda M,Takio K,Titani K,Utsumi H,Sasaki M,Inoue K J Cell Biol. 1991 Dec;115(6):1751-61 |
| Pubmed id:2065293 | Appearance and evolution of the specific chromosomal rearrangements associated with malignant transformation of mouse m5S cells. Kodama S,Okumura Y,Komatsu K,Sasaki MS Cancer Genet Cytogenet. 1991 Jun;53(2):185-97 |
| Pubmed id:2298824 | Mitogenic response of near-diploid mouse cell line m5S/1M induced by epidermal growth factor. Watanabe S,Umeda M,Nomura T,Kobayashi T,Nakano T,Arita H,Utsumi H,Sasaki MS,Inoue K J Cell Physiol. 1990 Jan;142(1):99-107 |
| Pubmed id:3108217 | The involvement of chromosome 13 in the X-ray-induced in vitro transformation of mouse m5S cells. Kodama S,Sasaki MS Jpn J Cancer Res. 1987 Apr;78(4):372-81 |
| Pubmed id:3571334 | Establishment and some mutational characteristics of 3T3-like near-diploid mouse cell line. Sasaki MS,Kodama S J Cell Physiol. 1987 Apr;131(1):114-22 |
| Pubmed id:6500229 | Integration of v-rasH does not necessarily transform an immortalized murine cell line. Tsunokawa Y,Esumi H,Sasaki MS,Mori M,Sakamoto H,Terada M,Sugimura T Gan. 1984 Sep;75(9):732-6 |
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LOT Information
| Cell No. | JCRB1322 | Cell Name | m5S |
|---|---|---|---|
| LOT No. | 10092008 | Lot Specification | distribution |
| Medium | alpha-MEM with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) | Temperature | 37 C |
| Cell Density at Seeding | 9.3x10^3 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.Split ratio (about 1/10). |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.7x10^6 |
| Viability at cell freezing (%) | 97.0 | Antibiotics Used | free |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |