JCRB1328 AR-EcoScreen

Cell information

Important Notice(s)

On the Distribution of AR-EcoScreen (JCRB1328)

JCRB1328 AR-EcoScreen細胞の分譲について

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1328 Cell Name   AR-EcoScreen
Profile   AR-EcoScreen is for evaluation of a rapid in vitro androgen receptor tarnscriptional activation assay. Other Name   
Animal   hamster, Chinese Strain   Chinese
Genus   Cricetulus Species   griseus
Sex    Age   
Identity   not done Tissue for Primary Cancer   ovary
Case history    Metastasis   No
Tissue Metastasized    Genetics   This cell is that stably express human androgen receptor, reportor gene (androgen response element linked to luciferase), and firefly luciferase gene.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   Transfected Established by   Iida, M.
Registered by   Araki, N. Regulation for Distribution   MTA between applicant and developer is required.
Comment    Year   2008
Medium   DMEM/F12 medium supplemented with 10% fetal bovine serum, hygromycin (25ug/mL) and zeocin (50 ug/mL). Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5% Tissue Sampling   Ovary
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8544
Reference
Pubmed id:15950433Screening for androgen receptor activities in 253 industrial chemicals by in vitro reporter gene assays using AR-EcoScreen cells.
Araki N,Ohno K,Nakai M,Takeyoshi M,Iida M
Toxicol In Vitro. 2005 Sep;19(6):831-42
Pubmed id:15713541Evaluation of a rapid in vitro androgen receptor transcriptional activation assay using AR-EcoScreen cells.
Araki N,Ohno K,Takeyoshi M,Iida M
Toxicol In Vitro. 2005 Apr;19(3):335-52
Pubmed id:15110108Study on anti-androgenic effects of bisphenol a diglycidyl ether (BADGE), bisphenol F diglycidyl ether (BFDGE) and their derivatives using cells stably transfected with human androgen receptor, AR-EcoScreen.
Satoh K,Ohyama K,Aoki N,Iida M,Nagai F
Food Chem Toxicol. 2004 Jun;42(6):983-93

Images



LOT Information

Cell No.   JCRB1328 Cell Name   AR-EcoScreen
LOT No.   01302009 Lot Specification   distribution
Medium   DMEM/F12(GIBCO)+hygromycin(25ug/ml)and Zeocin(50ug/ml) with 10% heat inactivated bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   1.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   NT
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1328 Cell Name   AR-EcoScreen
LOT No.   06122023 Lot Specification   distribution
Medium   DMEM/F12 with 25 ug/mL hygromycin, 50 ug/mL Zeocin, and 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs Cell Number in Vial (cells/1ml)   6.7 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)   98 Additional information   
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