JCRB1362 HCT-2
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1362 | Cell Name | HCT-2 |
|---|---|---|---|
| Profile | Hamster spleen lymphocytes transformed by human T-cell leukemia virus type I. | Other Name | |
| Animal | hamster, Syrian | Strain | Syrian |
| Genus | Mesocricetus | Species | auratus |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | Lethally irradiated MT-2(HTLV positive) cells were cocultivated with Syrian hamster cells in vitro. | Metastasis | |
| Tissue Metastasized | Genetics | HTLV-1 positive but HTLV-1 is not produced. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | The cells are forming loosely aggregated cell clumps. Hamster T-cell antigen positive. 44, XX(hamster). | |
| Classify | transformed | Established by | Akagi,T. |
| Registered by | Miyoshi.I. | Regulation for Distribution | |
| Comment | Year | 2007 | |
| Medium | RPMI 1640 medium with 20% fetal calf serum. | Methods for Passages | dilution |
| Cell Number on Passage | 1/2 - 1/3. | Race | |
| CO2 Conc. | 5% | Tissue Sampling | spleen |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:3009333 | Transformation of hamster spleen lymphocytes by human T-cell leukemia virus type I. Akagi T,Takata H,Ohtsuki Y,Takahashi K,Oka T,Yano S,Miyoshi I Int J Cancer. 1986 May 15;37(5):775-9 |
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LOT Information
| Cell No. | JCRB1362 | Cell Name | HCT-2 |
|---|---|---|---|
| LOT No. | 03012010 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 0.6-2.7x10^5 cells/ml | Methods for Passages | 1/2-1/3 dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 7.6x10^6 |
| Viability at cell freezing (%) | 95.9 | Antibiotics Used | free |
| Passage Number | p8* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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