JCRB9109 P3.6.2.8.1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9109 Cell Name   P3.6.2.8.1
Profile   myeloma, IgG1 secreting, (BALB/c mouse) Other Name   
Animal   mouse Strain   
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   IgG1 secreting myeloma
Life Span    Crisis PDL   
Morphology    Character   IgG1 secreting myeloma
Classify    Established by   Knopf,P.M. et al.
Registered by    Regulation for Distribution   
Comment   Prepared from TIB 8. Year   1987
Medium   DMEM + FCS 10% Methods for Passages   Dilution. Maintain cultures at cell concentrations between 10^5 and 10^6 per ml.
Cell Number on Passage   1-2 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3565
Reference
Pubmed id:4541304Retention of lymphocyte characteristics by myelomas and theta + -lymphomas: sensitivity to cortisol and phytohemagglutinin.
Ralph P
J Immunol. 1973 Jun;110(6):1470-5
Pubmed id:4317914Leukemia viruses associated with mouse myeloma cells.
Watson J,Ralph P,Sarkar S,Cohn M
Proc Natl Acad Sci U S A. 1970 Jun;66(2):344-51
Pubmed id:5439579Mouse myelomas and lymphomas in culture.
Horibata K,Harris AW
Exp Cell Res. 1970 Apr;60(1):61-77




LOT Information

Cell No.   JCRB9109 Cell Name   
LOT No.   041498 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1.5-3.0 x 10^5 cells/ml Methods for Passages   Dilution
Doubling Time    Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   75.0 Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
CLOSE