JCRB1404 Minami-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1404 Cell Name   Minami-1
Profile   follicular B cell lymphoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   39
Identity   available Tissue for Primary Cancer   
Case history   follicular lymphoma Metastasis   
Tissue Metastasized    Genetics   t(14;18)(q32;q21), bcl gene rearrangement
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   
Classify   tumor Established by   Asou, H.
Registered by   Asou, H. Regulation for Distribution   
Comment    Year   2009
Medium   RPMI1640 medium with 10% featal bovine serum. Methods for Passages   dilution (split ratio 1:2)
Cell Number on Passage   0.5-2.0 x 10^6 cells Race   
CO2 Conc.   5% Tissue Sampling   lymphonode
Tissue Type   tumor

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8528
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LOT Information

Cell No.   JCRB1404 Cell Name   Minami-1
LOT No.   05302011 Lot Specification   distribution2
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.02-4.64x10^5 cells/ml Methods for Passages   Dilution (split ratio 1:2)
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.36x10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:8,10
D7S820:8,10
D16S539:9
VWA:16,18
TH01:7,9.3
AM:X
TPOX:11
CSF1PO:10,11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1404 Cell Name   Minami-1
LOT No.   04192011 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.51-10.3x10^5 cells/ml Methods for Passages   Dilution (split ratio 1:2)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.82x10^6
Viability at cell freezing (%)   86.0 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:8,10
D7S820:8,10
D16S539:9
VWA:16,18
TH01:7,9.3
AM:X
TPOX:11
CSF1PO:10,11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1404 Cell Name   Minami-1
LOT No.   10222020 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.4-3.3x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   96.40 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:8,10
D7S820:8,10
D16S539:9
VWA:16,18
TH01:7,9.3
AM:X
TPOX:11
CSF1PO:10,11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   58.2 Additional information   This cell line is week for freeze and thaw, please start small volume culture (2 or 3 ml)
Images
Cell No.   JCRB1404 Cell Name   Minami-1
LOT No.   10232020 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   2.8-3.3x10^5 cell/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:8,10
D7S820:8,10
D16S539:9
VWA:16,18
TH01:7,9.3
AM:X
TPOX:11
CSF1PO:10,11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   54.4 Additional information   This cell line is week for freeze and thaw, please start small volume culture (2 or 3 ml)
Images
Cell No.   JCRB1404 Cell Name   Minami-1
LOT No.   11122020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012, Lot 12J396) Temperature   37 C
Cell Density at Seeding   2.5 - 8.5 x 10^5 cells/mL Methods for Passages   Simple dilution, split ratio = 1/2. In case the growth is poor, add 20% FBS and 5 ug/mL insulin. The size of cells is very small. When centrifuge the cells, 1200 rpm for 5 min is required.
Doubling Time   very slow Cell Number in Vial (cells/1ml)   6.2 x 10^6
Viability at cell freezing (%)   68.9 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   68.9 Additional information   
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