JCRB1417 KYSE960

Cell information

Important Notice(s)

KYSEシリーズ細胞株の民間企業への分譲について

KYSEシリーズ細胞株の使用について

Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1417 Cell Name   KYSE960
Profile   Human moderately differentiated squamous cell carcinoma cell line established from esophageal cancer. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   58
Identity   available Tissue for Primary Cancer   esophagus
Case history   moderately differentiated squamous cell carcinoma, stage 3 Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   T3M1N0 stage 3
Classify   tumor Established by   Shimada, Y.
Registered by   Shimada, Y. Regulation for Distribution   
Comment    Year   2011
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 2% fetal calf serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage   split ratio = 1/5 Race   
CO2 Conc.   5% Tissue Sampling   esophagus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8512
Reference
Pubmed id:17935131Gankyrin oncoprotein overexpression as a critical factor for tumor growth in human esophageal squamous cell carcinoma and its clinical significance.
Ortiz CM,Ito T,Tanaka E,Tsunoda S,Nagayama S,Sakai Y,Higashitsuji H,Fujita J,Shimada Y
Int J Cancer. 2008 Jan 15;122(2):325-32
Pubmed id:16951226The biological role of the low-affinity p75 neurotrophin receptor in esophageal squamous cell carcinoma.
Okumura T,Tsunoda S,Mori Y,Ito T,Kikuchi K,Wang TC,Yasumoto S,Shimada Y
Clin Cancer Res. 2006 Sep 1;12(17):5096-103
Pubmed id:16003725Cisplatin-dependent upregulation of death receptors 4 and 5 augments induction of apoptosis by TNF-related apoptosis-inducing ligand against esophageal squamous cell carcinoma.
Kondo K,Yamasaki S,Sugie T,Teratani N,Kan T,Imamura M,Shimada Y
Int J Cancer. 2006 Jan 1;118(1):230-42
Pubmed id:17179732Gli-1 expression is associated with lymph node metastasis and tumor progression in esophageal squamous cell carcinoma.
Mori Y,Okumura T,Tsunoda S,Sakai Y,Shimada Y
Oncology. 2006;70(5):378-89
Pubmed id:17671398ABCG2 expression is an independent unfavorable prognostic factor in esophageal squamous cell carcinoma.
Tsunoda S,Okumura T,Ito T,Kondo K,Ortiz C,Tanaka E,Watanabe G,Itami A,Sakai Y,Shimada Y
Oncology. 2006;71(3-4):251-8
Pubmed id:15814639Prognostic significance of fascin overexpression in human esophageal squamous cell carcinoma.
Hashimoto Y,Ito T,Inoue H,Okumura T,Tanaka E,Tsunoda S,Higashiyama M,Watanabe G,Imamura M,Shimada Y
Clin Cancer Res. 2005 Apr 1;11(7):2597-605
Pubmed id:14522385Growth inhibition through activation of peroxisome proliferator-activated receptor gamma in human oesophageal squamous cell carcinoma.
Hashimoto Y,Shimada Y,Itami A,Ito T,Kawamura J,Kawabe A,Kaganoi J,Maeda M,Watanabe G,Imamura M
Eur J Cancer. 2003 Oct;39(15):2239-46
Pubmed id:14522885Identification of ZASC1 encoding a Krüppel-like zinc finger protein as a novel target for 3q26 amplification in esophageal squamous cell carcinomas.
Imoto I,Yuki Y,Sonoda I,Ito T,Shimada Y,Imamura M,Inazawa J
Cancer Res. 2003 Sep 15;63(18):5691-6
Pubmed id:12538476Cell culture in esophageal squamous cell carcinoma and the association with molecular markers.
Shimada Y,Maeda M,Watanabe G,Yamasaki S,Komoto I,Kaganoi J,Kan T,Hashimoto Y,Imoto I,Inazawa J,Imamura M
Clin Cancer Res. 2003 Jan;9(1):243-9
Pubmed id:10591495Ovine pestiviruses: their taxonomic status revealed by palindromic nucleotide substitutions.
Giangaspero M,Harasawa R
Vet Microbiol. 1999 Oct;70(1-2):33-9
Pubmed id:9033652Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K
Int J Cancer. 1997 Feb 7;70(4):437-42

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LOT Information

Cell No.   JCRB1417 Cell Name   KYSE960
LOT No.   07202011 Lot Specification   distribution
Medium   RPMI1640(GIBCO) and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.1-4.9x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA(Split ratio=1/5).
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.61x10^6
Viability at cell freezing (%)   99.4 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:11
D7S820:10,12
D16S539:10,11,12
VWA:16,17
TH01:6
AM:X
TPOX:11
CSF1PO:10,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1417 Cell Name   KYSE960
LOT No.   10072016 Lot Specification   distribution
Medium   Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 5% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.4 - 2.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   65.7 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   65.7 Additional information   
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