JCRB1557 KKU-213

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1557 Cell Name   KKU-213
profile   Human mixed (papillary and non-papillary) cholangiocarcinoma cell line established from biliary tract. Alias   KKU-M213
animal   human strain   
genus   Homo species   sapiens
sex   M age   58-year-old
identity   available tissue   
case history    metastasis   
metastorgn    genetics   
lifespan   infinite crisispdl   
morphology   epithelial-like charact   Mixed papillary and non-papillary cholangiocarcinoma
classify   tumor originator   Sripa, B.
register   Okada, S. regulation   Acknowledgment is requested
comment   In publishing the research results to be obtained by use of the cell lines, an acknowledgment to the Dr. Banchob Spira (Khon Kaen University) is requested. dyear   2013
medium   DMEM with 10% Fetal bovine serum. pasmethod   Cells harvested after treatment with trypsin and EDTA.
pascellno   ? race   
co2conc   5% tohroku   registered
preporgn   biliary tract vrsummary   tested                [-/negative, +/positive, nt/not tested, GAPDH = positive control]
vrsdetect   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) tissueform   





LOT Information

Cell No.   JCRB1557 Cell Name   KKU-213
LOT No.   10282013 LOTSPEC   distribution
MEDIUM   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) GROWTEMP   37 C
PASCELLNO   1.1-7.8x10^4 cells/sq.cm PASMETHOD   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
GROWTHRATE   NT FREEZCONC   1.5x10^6
VIABLFREEZ   99.5 ANTIBIOTIC   free
PASSAGE   p9* PDL   NT
MYCO   - BACT   -
FUNGI   - ISOZYME   NT
CHRMODE   NT CHRDISTRB   NT
SURFACEAG   NT DNA Profile (STR)   D5S818:9
D13S317:8,12
D7S820:11
D16S539:9,11
VWA:18
TH01:7
AM:X
TPOX:8
CSF1PO:9,13
ANCHORDEPD   Yes EXOGENE   NT
FMEDIUM   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2   5 %
RFLP   NT FROMLOT   deposit
Additional information   


Cell No.   JCRB1557 Cell Name   KKU-213
LOT No.   05242016 LOTSPEC   distribution
MEDIUM   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) GROWTEMP   37 C
PASCELLNO   1.4-3.8x10^4 cells/sq.cm PASMETHOD   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
GROWTHRATE   NT FREEZCONC   1.1x10^6
VIABLFREEZ   98.4 ANTIBIOTIC   free
PASSAGE   p9* PDL   NT
MYCO   - BACT   -
FUNGI   - ISOZYME   NT
CHRMODE   NT CHRDISTRB   NT
SURFACEAG   NT DNA Profile (STR)   D5S818:9
D13S317:8,12
D7S820:11
D16S539:9,11
VWA:18
TH01:7
AM:X
TPOX:8
CSF1PO:9,13
ANCHORDEPD   Yes EXOGENE   NT
FMEDIUM   BAMBANKER(LYMPHOTEC Inc.,CS-0 2-001,NIPPON Genetics Co., LTD) CO2   5%
RFLP    FROMLOT   10182013(seed)
Additional information   On vial, labeled as KKU-M213.


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