JCRB1568 KKU-100

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1568 Cell Name   KKU-100
profile   Human poorly differentiated cholangiocarcinoma cell line established from biliary tract. Alias   
animal   human strain   
genus   Homo species   sapiens
sex   F age   65-year-old
identity   available tissue   
case history    metastasis   
metastorgn    genetics   
lifespan   infinite crisispdl   
morphology   epithelial-like charact   poorly differentiated cholangiocarcinoma
classify   tumor originator   Spira, B.
register   Okada, S. regulation   Acknowledgment is requested
comment   In publishing the research results to be obtained by use of the cell lines, an acknowledgment to the Dr. Banchob Spira (Khon Kaen University) is requested. dyear   2013
medium   DMEM with 10% Fetal bovine serum. pasmethod   Cells harvested after treatment with trypsin and EDTA.
pascellno   ? race   
co2conc   5% tohroku   registered
preporgn   biliary tract vrsummary   tested                [-/negative, +/positive, nt/not tested, GAPDH = positive control]
vrsdetect   GAPDH(+), CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), HBV(-), parvoB19(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) tissueform   


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LOT Information

Cell No.   JCRB1568 Cell Name   KKU-100
LOT No.   01312014 LOTSPEC   distribution
MEDIUM   DMEM with 10 % heat inactivated fetal bovine serum(SIGMA027K03911) GROWTEMP   37 C
PASCELLNO   0.78-3.33x10^4cells/sq.cm PASMETHOD   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
GROWTHRATE   NT FREEZCONC   1.13x10^6
VIABLFREEZ   92.1 ANTIBIOTIC   free
PASSAGE   p9* PDL   NT
MYCO   - BACT   -
FUNGI   - ISOZYME   NT
CHRMODE   NT CHRDISTRB   NT
SURFACEAG   NT DNA Profile (STR)   D5S818:11,13
D13S317:9
D7S820:8,11
D16S539:10,11
VWA:17,19
TH01:8,9
AM:X
TPOX:8,9
CSF1PO:11,13
ANCHORDEPD   Yes EXOGENE   NT
FMEDIUM   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2   5 %
RFLP   NT FROMLOT   12242013
Additional information   


Cell No.   JCRB1568 Cell Name   KKU-100
LOT No.   07122016 LOTSPEC   distribution
MEDIUM   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) GROWTEMP   37 C
PASCELLNO   3.4 - 7.3 x 10^4 cells/mL PASMETHOD   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
GROWTHRATE   approx. 33 hrs. FREEZCONC   2.4 x 10^6
VIABLFREEZ   98.4 ANTIBIOTIC   free
PASSAGE   Unknown (11 at bank) PDL   
MYCO   - BACT   -
FUNGI   - ISOZYME   
CHRMODE    CHRDISTRB   
SURFACEAG    DNA Profile (STR)   
ANCHORDEPD   Yes EXOGENE   
FMEDIUM   10% DMSO, 20% FBS - DMEM CO2   5%
RFLP    FROMLOT   01212014
Additional information   


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