JCRB1582 TWNT-1
Cell information
Important Notice(s)This cell line is available for only academic users.
Articles regarding TMNK-1 / TWNT-1 / MMNK-1
Cell type：genetically-modified cells (View Pricing Information)
| JCRB No. | JCRB1582 | Cell Name | TWNT-1 |
|---|---|---|---|
| Profile | Immortalized human hepatic stellate cell line (established from LI90). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | liver |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | Expressing catalytic subunit of human telomerase reverse transcriptase (hTERT) and enhanced green fluorescent protein (EGFP). | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | |
| Classify | transformed | Established by | Kobayashi, N. |
| Registered by | Mochitate, K. | Regulation for Distribution | [Murakami et al., Lab Invest. 72: 731-739, 1995] should be cited at the paper submission. |
| Comment | The distribution is limited to academic Japan domestic users and academic oversea users. The following reference should be cited at the publication of papers. Murakami et al. Lab Invest. 72: 731-739, 1995. | Year | 2013 |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum. | Methods for Passages | 0.05% trypsin and 0.53 mM EDTA. split ratio = 1/3 - 1/4. |
| Cell Number on Passage | 1.5 - 6.0 x 10^4 cells/sq.cm | Race | |
| CO2 Conc. | 5% | Tissue Sampling | LI90 cell line |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:12811248 | Establishment of immortalized human hepatic stellate scavenger cells to develop bioartificial livers. Watanabe T,Shibata N,Westerman KA,Okitsu T,Allain JE,Sakaguchi M,Totsugawa T,Maruyama M,Matsumura T,Noguchi H,Yamamoto S,Hikida M,Ohmori A,Reth M,Weber A,Tanaka N,Leboulch P,Kobayashi N Transplantation. 2003 Jun 15;75(11):1873-80 |
| Pubmed id:7540235 | Establishment of a new human cell line, LI90, exhibiting characteristics of hepatic Ito (fat-storing) cells. Murakami K,Abe T,Miyazawa M,Yamaguchi M,Masuda T,Matsuura T,Nagamori S,Takeuchi K,Abe K,Kyogoku M Lab Invest. 1995 Jun;72(6):731-9 |
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LOT Information
| Cell No. | JCRB1582 | Cell Name | TWNT-1 |
|---|---|---|---|
| LOT No. | 04082014 | Lot Specification | distribution |
| Medium | DMEM with 10% fetal bovine serum(SIGMA12J396,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 1.5-6.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin and 0.53mM EDTA.Split ratio=1/2-1/4 |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.37x10^6 |
| Viability at cell freezing (%) | 95.3 | Antibiotics Used | |
| Passage Number | p16 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,13 D13S317:12 D7S820:10,11 D16S539:9,10 VWA:16,17 TH01:9 AM:X TPOX:8,11 CSF1PO:9,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1582 | Cell Name | TWNT-1 |
|---|---|---|---|
| LOT No. | 10272017 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | approx. 3 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 32 hrs. | Cell Number in Vial (cells/1ml) | 1.6 x 10^6 |
| Viability at cell freezing (%) | 86.6 | Antibiotics Used | free |
| Passage Number | P18 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 86.6 | Additional information |