JCRB1956 HSC-39 PF
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1956 | Cell Name | HSC-39 PF |
|---|---|---|---|
| Profile | Cell line that can be cultured only with HamF12/DMEM (protein free). Culture start at thawing must with 2% FBS. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 54-year-old |
| Identity | available | Tissue for Primary Cancer | stomach |
| Case history | scirrhous stomach cancer | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | round shape | Character | floating |
| Classify | tumor | Established by | Yanagihara Kazuyoshi |
| Registered by | Yuki Iino | Regulation for Distribution | |
| Comment | Year | ||
| Medium | HamF12/DMEM medium (serum free) Culture start at thawing must with 2% FBS. | Methods for Passages | dilution |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5% | Tissue Sampling | ascites |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB1956 | Cell Name | HSC-39 PF |
|---|---|---|---|
| LOT No. | 11022023 | Lot Specification | distribution |
| Medium | DMEM/ F12(1/1) medium (GIBCO 11320-033) with 0% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396). At thawing, start the culture with 2% FBS and gradually decrease FBS concentration. | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | Dilution (ratio:2-10- x) | |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.1x10^6 |
| Viability at cell freezing (%) | 90.30 | Antibiotics Used | free |
| Passage Number | ｐ19* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:13,14 D13S317:9 D7S820:12 D16S539:9 VWA:14 TH01:7,9 AM:X TPOX:8 CSF1PO:11 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 64.6 | Additional information | Culture start at thawing must with 2% FBS with 25T flask. It is possiple to subculture 10x-100x dilution for single cell culture. |
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