JCRB1963 UCCA-21
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1963 | Cell Name | UCCA-21 |
|---|---|---|---|
| Profile | Derived from colorectal cancer with ulcerative colitis. Isolated as well-differentiated adenocarcinoma by cloning. Paneth cells included. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 33-year-old |
| Identity | available | Tissue for Primary Cancer | Rectum moderately adenocarcinoma |
| Case history | Duration of UC 11years | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | DT=63.6 hrs |
| Classify | tumor | Established by | Kazuya YAMASHITA |
| Registered by | YAMASHITA Kazuya | Regulation for Distribution | |
| Comment | Year | ||
| Medium | ES medium with 15% FBS (at the establishment of cell line). Current medium for propagation is Eagle's minimum essential medium with 10% FBS. | Methods for Passages | 0.25%Trypsin+0.02%EDTA, passage 1:5split /1 week |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | primary rectum carcinoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:16206288 | Disruption of the p53-p53r2 DNA repair system in ulcerative colitis contributes to colon tumorigenesis. Yoshida T,Haga S,Numata Y,Yamashita K,Mikami T,Ogawa T,Ohkusa T,Okayasu I Int J Cancer. 2006 Mar 15;118(6):1395-403 |
| Pubmed id:15016319 | Unique characteristics of rectal carcinoma cell lines derived from invasive carcinomas in ulcerative colitis patients. Yamashita K,Yasuda S,Kuba T,Otani Y,Fujiwara M,Okayasu I Cancer Sci. 2004 Mar;95(3):211-7 |
| Pubmed id:none | 細胞株を用いた免疫組織化学染色マルチプルコントロール試料の作製と標準化の基礎的検討. 山下和也, 他． 神奈川県臨床検査技師会雑誌(2188-6628) |
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LOT Information
| Cell No. | JCRB1963 | Cell Name | UCCA-21 |
|---|---|---|---|
| LOT No. | 08252023 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium (GIBCO cat No. 11095-080) with 10% fetal bovine serum (not HI) (JRH BIOSCIENCES Lot#2G1937) | Temperature | 37 C |
| Cell Density at Seeding | 3.6-9.2x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin (GIBCO) and 0.02% EDTA. (Split ratio=1/3-1/5) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 7.8x10^6 |
| Viability at cell freezing (%) | 99.60 | Antibiotics Used | free |
| Passage Number | p7* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:8 D7S820:11,12 D16S539:10 VWA:15 TH01:9 AM:X TPOX:8 CSF1PO:10,11 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 86.2 | Additional information | We recommend to start culture with 25T Flask. |
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