JCRB1970 MDCK-QKO

Cell information

Important Notice(s)

Crispr/Cas9法を用いてゲノム編集が施された細胞株の分譲について

About the distribution of cell lines developed by Crispr/Cas9 technology.

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1970 Cell Name   MDCK-QKO
Profile   Epidermal growth factor (EGF), heparin-binding EGF (HBEGF), transforming growth factor alpha (TGFalpha), and epiregulin (EREG)-quadruple deficient MDCK cell line. Other Name   
Animal   dog Strain   
Genus   Canis Species   familiaris
Sex   F Age   
Identity   not done Tissue for Primary Cancer   
Case history   normal Metastasis   
Tissue Metastasized    Genetics   EGF, HBEGF, TGFalpha, EREG quadruple deficient
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Shuhao Lin
Registered by   Michiyuki MATSUDA Regulation for Distribution   non-profit, academic research purposes only
Comment    Year   
Medium   DMEM with 10% FBS Methods for Passages   0.25% trypsin + 0.02% EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Reference
Pubmed id:34667080Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration.
Lin S,Hirayama D,Maryu G,Matsuda K,Hino N,Deguchi E,Aoki K,Iwamoto R,Terai K,Matsuda M
Life Sci Alliance. 2022 Jan;5(1):

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LOT Information

Cell No.   JCRB1970 Cell Name   MDCK-QKO
LOT No.   09132023 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (GIBCO cat No. 11885) with 10% fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   7.28x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.22x10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
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