JCRB4003 KE-43
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB4003 | Cell Name | KE-43 |
|---|---|---|---|
| Profile | moderately differentiated adenocarcinoma established from primary tumor. Transition to poorly differentiated adenocarcinoma by passage. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 54-year-old |
| Identity | available | Tissue for Primary Cancer | colon |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | KRAS G12V hetero, 42 XX | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Production of CEA, CA19-9 and laminin, DT=33.8 hrs |
| Classify | tumor | Established by | Kazuya YAMASHITA |
| Registered by | YAMASHITA Kazuya | Regulation for Distribution | |
| Comment | Year | ||
| Medium | MEM+10%FBS | Methods for Passages | 0.25%Trypsin+0.02%EDTA, passage 1:20split /1 week |
| Cell Number on Passage | 2.5 x 10^5 cells/25cm^2 | Race | |
| CO2 Conc. | 5% | Tissue Sampling | primary colonic carcinoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:16206288 | Disruption of the p53-p53r2 DNA repair system in ulcerative colitis contributes to colon tumorigenesis. Yoshida T,Haga S,Numata Y,Yamashita K,Mikami T,Ogawa T,Ohkusa T,Okayasu I Int J Cancer. 2006 Mar 15;118(6):1395-403 |
| Pubmed id:15016319 | Unique characteristics of rectal carcinoma cell lines derived from invasive carcinomas in ulcerative colitis patients. Yamashita K,Yasuda S,Kuba T,Otani Y,Fujiwara M,Okayasu I Cancer Sci. 2004 Mar;95(3):211-7 |
| Pubmed id:1390419 | [Establishment and characterization of a human colonic cancer cell line expressing carbohydrate antigen 19-9 and carcinoembryonic antigen]. Atari E,Watanabe K,Yamashita K Hum Cell. 1992 Jun;5(2):174-9 |
| Pubmed id:none | 細胞株を用いた免疫組織化学染色マルチプルコントロール試料の作製と標準化の基礎的検討. 山下和也, 他． 神奈川県臨床検査技師会雑誌(2188-6628) |
| Pubmed id:none | ヒト大腸癌培養細胞株の腹腔内増殖能と癌関連遺伝子および癌関連蛋白異常に関する検討． 西山保比古、他． 日本大腸肛門病学会雑誌、50: 562- 571, 1997. |
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LOT Information
| Cell No. | JCRB4003 | Cell Name | KE-43 |
|---|---|---|---|
| LOT No. | 02212024 | Lot Specification | distribution |
| Medium | MEM (GIBCO cat.No.11095-080) with 20% fetal bovine serum ((SIGMA, Cat#172012, Lot#12J396, non HI ) | Temperature | 37 C |
| Cell Density at Seeding | 0.0453-2.98x10^5 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin (GIBCO) and 0.02% EDTA in PBS (-). |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.96x10^6 |
| Viability at cell freezing (%) | 79.90 | Antibiotics Used | free |
| Passage Number | p206 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,12 D13S317:9 D7S820:8,10 D16S539:9,11 VWA:14,18 TH01:6 AM:X TPOX:8 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 89.72 | Additional information |