IFO50069 Alexander cells

Cell information

Important Notice(s)

The Alexander cells: another name of PLC/PRF/5 cell line.

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50069 Cell Name   Alexander cells
Profile   hepatoma, HBs antigen-positive. This cell line is the same cell line as PLF/PRF/5 (other name of PLC/PRF/5). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   24
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   malignant liver cancer Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   hepatoma, HBs antigen-positive
Classify   tumor Established by   Alexander, J. J.
Registered by   Takeuchi,M. Regulation for Distribution   
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV + HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes HB virus genome fragment was detected by realtime PCR. However, viral particles were not found (Br. J. Cancer, 34: 509 515 (1976)) in this cell line. In addition, this cell line has not been reported to produce HBV. The infectious HBV particles will not be produced by this cell line.

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0485
Reference
Pubmed id:63998Establishment of a continuously growing cell line from primary carcinoma of the liver.
Alexander JJ,Bey EM,Geddes EW,Lecatsas G
S Afr Med J. 1976 Dec 18;50(54):2124-8
Pubmed id:187208Hepatitis B surface antigen produced by a human hepatoma cell line.
MacNab GM,Alexander JJ,Lecatsas G,Bey EM,Urbanowicz JM
Br J Cancer. 1976 Nov;34(5):509-15

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LOT Information

Cell No.   IFO50069 Cell Name   Alexander cells
LOT No.   1011 Lot Specification   distribution
Medium   RPMI 1640, 90% ; FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   6 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type A), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50069 Cell Name   Alexander cells
LOT No.   10252013 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099). Temperature   37C
Cell Density at Seeding   3.5 - 4.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25%trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.9 x 10^6 (cells/1
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeA),NP,LDH were examined. Human
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:11,12
D7S820:9,11
D16S539:13
VWA:15,16
TH01:7,8
AM:X
TPOX:8
CSF1PO:10
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   89.0 Additional information   
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