IFO50271 HUV-EC-C
Cell information
Important Notice(s)IFO50271 HUV-EC-Cのウイルス検査成績について
Cell type:general cells (View Pricing Information)
JCRB No. | IFO50271 | Cell Name | HUV-EC-C |
---|---|---|---|
Profile | Normal endothelial cells with limited life span. For cultivation, endothelial cell growth supplement can be substituted by ca 10 ng/ml basic FGF. | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | Age | ||
Identity | available | Tissue for Primary Cancer | vascular system |
Case history | Metastasis | ||
Tissue Metastasized | Genetics | ||
Life Span | finite | Crisis PDL | 50-60 |
Morphology | endothelial | Character | normal endothelial cell, umbilical cord |
Classify | primary | Established by | Hoshi, H. |
Registered by | Suzuki,N. | Regulation for Distribution | |
Comment | Year | 1990 | |
Medium | MCDB 107 with 10% FBS, 100ug/ml heparin and 50ug/ml endothelial cell growth supplement | Methods for Passages | trypsin-EDTA |
Cell Number on Passage | Race | ||
CO2 Conc. | 5 % | Tissue Sampling | umbilical cord, vein |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | + | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes | The HSV6B genome fragment was detected by realtime PCR. However, the copy number of HSV6B integrated to host genome was one and unchanged during passages. In addition, HSV6 viral replication was not observed even by the stimulation with chemicals. JCRB judged that HHV6 production by HUV-EC-C will not occur. Reference: Cytotechnology,70: 141-152 (2018) |
Reference | |
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Pubmed id:28755030 | The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture. Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A Cytotechnology. 2018 Feb;70(1):141-152 |
Pubmed id:8999915 | Characterization of the binding of serum amyloid P to laminin. Zahedi K J Biol Chem. 1997 Jan 24;272(4):2143-8 |
Pubmed id:8621443 | Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-encoded domain. Soker S,Fidder H,Neufeld G,Klagsbrun M J Biol Chem. 1996 Mar 8;271(10):5761-7 |
Pubmed id:6333682 | Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Hoshi H,McKeehan WL Proc Natl Acad Sci U S A. 1984 Oct;81(20):6413-7 |
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LOT Information
- 05282012
- 05232011
- 06152010
- 03092009
- 03042008
- 10312006
- 06092005
- 06252001
- 1127
- 10142003
- 03302015
- 10192019
- 08272018
- 05232023
Cell No. | IFO50271 | Cell Name | |
---|---|---|---|
LOT No. | 05282012 | Lot Specification | distribution |
Medium | Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). | Temperature | 37C |
Cell Density at Seeding | 3 - 6 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
Doubling Time | approx. 39 hrs | Cell Number in Vial (cells/1ml) | 7.9 x 10^5 |
Viability at cell freezing (%) | 97.5 | Antibiotics Used | free |
Passage Number | P25 | PDL | +13.3 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50271 | Cell Name | |
---|---|---|---|
LOT No. | 05232011 | Lot Specification | distribution |
Medium | Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). | Temperature | 37 C |
Cell Density at Seeding | 4.0 - 6.4 x 10^4 cells/mL | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. |
Doubling Time | approx. 2 days | Cell Number in Vial (cells/1ml) | 1.0 x 10^6 |
Viability at cell freezing (%) | 96.2 | Antibiotics Used | free |
Passage Number | P25 | PDL | +13 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50271 | Cell Name | |
---|---|---|---|
LOT No. | 06152010 | Lot Specification | distribution |
Medium | Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). | Temperature | 37 C |
Cell Density at Seeding | 6.6 - 8.4 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. |
Doubling Time | approx. 2 days | Cell Number in Vial (cells/1ml) | 6.3 x 10^5 |
Viability at cell freezing (%) | 82.5 | Antibiotics Used | free |
Passage Number | P25 | PDL | +10.6 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50271 | Cell Name | |
---|---|---|---|
LOT No. | 03092009 | Lot Specification | distribution |
Medium | Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). | Temperature | 37 C |
Cell Density at Seeding | 5 - 6 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS. |
Doubling Time | 46 hrs | Cell Number in Vial (cells/1ml) | 6.6 x 10^5 (viable) |
Viability at cell freezing (%) | 88.2 | Antibiotics Used | free |
Passage Number | P25 | PDL | +13.5 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50271 | Cell Name | |
---|---|---|---|
LOT No. | 03042008 | Lot Specification | distribution |
Medium | Ham's F12K medium with 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplment (ECGS) and 10% fetal bovine serum (GIBCO Cat#10099, lot 484375) | Temperature | 37 C |
Cell Density at Seeding | approx. 7 x 10^4 cells/ml (1.4 x 10^4 cells/cm2) | Methods for Passages | 0.25% trypsin and 0.02% EDTA. Split ratio 1:4. |
Doubling Time | D.T. 2 - 3 days | Cell Number in Vial (cells/1ml) | 6.7 x 10^5 |
Viability at cell freezing (%) | 89 | Antibiotics Used | free |
Passage Number | P25 | PDL | +13.5 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50271 | Cell Name | |
---|---|---|---|
LOT No. | 10312006 | Lot Specification | distribution |
Medium | Ham's F12K medium with 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (Sigma) and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) | Temperature | 37 C |
Cell Density at Seeding | 5 - 10 x 10^4 cells/ml | Methods for Passages | 0.05-0.25% trypsin and 0.02% EDTA |
Doubling Time | 1.5 - 2.0 days | Cell Number in Vial (cells/1ml) | 8.9 x 10^5 |
Viability at cell freezing (%) | 94.3 | Antibiotics Used | free |
Passage Number | P25 | PDL | +14.1 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD, AST. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50271 | Cell Name | |
---|---|---|---|
LOT No. | 06092005 | Lot Specification | distribution |
Medium | Ham's F12K medium with 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement and 10% fetal bovine serum (FBS lot; GIBCO 4218333S) | Temperature | 37 C |
Cell Density at Seeding | 1 - 2 x 10^5 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
Doubling Time | 30 - 40 hrs | Cell Number in Vial (cells/1ml) | 7.7 x 10^5 |
Viability at cell freezing (%) | 82 | Antibiotics Used | free |
Passage Number | P26 | PDL | 13 at bank |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - culture medium | CO2 Conc. | 5% |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50271 | Cell Name | |
---|---|---|---|
LOT No. | 06252001 | Lot Specification | distribution |
Medium | Ham's F12K medium with 50 ug/ml ECGS (Sigma E2759), 100 ug/ml heparin and 10% FCS | Temperature | 37 C |
Cell Density at Seeding | 2-6 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA. Growth at least additional 11 PDL was confirmed at bank. |
Doubling Time | Cell Number in Vial (cells/1ml) | 5.8 x 10^5 | |
Viability at cell freezing (%) | 77.1 | Antibiotics Used | free |
Passage Number | P25 | PDL | 12 at bank |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD, AST. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Exoteric Gene | ||
Medium for Freezing | CO2 Conc. | ||
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50271 | Cell Name | HUV-EC-C |
---|---|---|---|
LOT No. | 1127 | Lot Specification | distribution |
Medium | Ham's F12K with 10% FBS, 100 ug/ml heparin-Ca, and 50 ug/ml ECGS. | Temperature | 37 C |
Cell Density at Seeding | 5 x 10^4 cells/ml | Methods for Passages | trypsin-EDTA |
Doubling Time | 4 folds/week | Cell Number in Vial (cells/1ml) | 4.6 x 10^5 |
Viability at cell freezing (%) | 84.9 | Antibiotics Used | free |
Passage Number | P24 | PDL | +12.4 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | NP, G6PD (type B), MD, AST |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Exoteric Gene | ||
Medium for Freezing | 10% DMSO, 20% serum (final conc.)-culture medium | CO2 Conc. | |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50271 | Cell Name | |
---|---|---|---|
LOT No. | 10142003 | Lot Specification | distribution |
Medium | Ham's F12K medium with 10% fetal bovine serum, 50 ug/ml ECGS, 100 ug/ml heparin-Na salt (FBS lot; GIBCO 3762782S) | Temperature | 37 C |
Cell Density at Seeding | 5-6 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
Doubling Time | D.T. = 2 days (max) | Cell Number in Vial (cells/1ml) | 4.1 x 10^5 |
Viability at cell freezing (%) | 80.3 | Antibiotics Used | free |
Passage Number | P25 | PDL | +12.4 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST, LD. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - culture meidum | CO2 Conc. | 5% |
Viability immediately after thawing (%) | Additional information |
Cell No. | IFO50271 | Cell Name | HUV-EC-C |
---|---|---|---|
LOT No. | 03302015 | Lot Specification | distribution |
Medium | Ham's F12K medium with 10% fetal bovine serum (FBS; GIBCO Cat.# 10091), 100 ug/ml heparin and 50 ug/ml endothelial cell growth supplement | Temperature | 37 C |
Cell Density at Seeding | 2.4 - 5.1 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
Doubling Time | approx. 1.5 day | Cell Number in Vial (cells/1ml) | 8.7 x 10^5 |
Viability at cell freezing (%) | 86 | Antibiotics Used | free |
Passage Number | P25 | PDL | +12.8 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD, MD. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | D5S818:11,12 D13S317:9,11 D7S820:8,12 D16S539:11,12 VWA:16 TH01:6,9.3 AM:X TPOX:8,11 CSF1PO:11,12 |
|
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - Ham's F12K | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 86 | Additional information |
Cell No. | IFO50271 | Cell Name | HUV-EC-C |
---|---|---|---|
LOT No. | 10192019 | Lot Specification | distribution |
Medium | Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). | Temperature | 37 C |
Cell Density at Seeding | 5.3 - 7.1 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
Doubling Time | approx. 43 hrs | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
Viability at cell freezing (%) | 87.9 | Antibiotics Used | free |
Passage Number | P24 | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - Ham's F12K | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 87.9 | Additional information |
Cell No. | IFO50271 | Cell Name | HUV-EC-C |
---|---|---|---|
LOT No. | 08272018 | Lot Specification | distribution |
Medium | Ham's F12K medium with 10% FBS, 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). | Temperature | 37 C |
Cell Density at Seeding | 3 - 7 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
Doubling Time | approx. 2.5 days | Cell Number in Vial (cells/1ml) | 9.0 x 10^5 |
Viability at cell freezing (%) | 96 | Antibiotics Used | free |
Passage Number | P24 | PDL | +14.7 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - Ham's F12K | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 90 | Additional information |
Cell No. | IFO50271 | Cell Name | HUV-EC-C |
---|---|---|---|
LOT No. | 05232023 | Lot Specification | distribution |
Medium | Ham's F12K medium with 10% FBS (GIBCO Cat. # 10437, Lot 1072322), 100 ug/ml heparin, 50 ug/ml endothelial cell growth supplement (ECGS). ECGS can be substituted by 10 ng/ml bFGF (FGF-2). | Temperature | 37 C |
Cell Density at Seeding | 2.4 - 9.6 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
Doubling Time | 26 - 76 hrs. | Cell Number in Vial (cells/1ml) | 1.7 x 10^6 |
Viability at cell freezing (%) | 96.8 | Antibiotics Used | free |
Passage Number | P24 | PDL | +14.7 |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | D5S818:11,12 D13S317:9,11 D7S820:8,12 D16S539:11,12 VWA:16 TH01:6,9.3 AM:X TPOX:8,11 CSF1PO:11,12 |
|
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - Ham's F12K | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 95.7 | Additional information |