JCRB0065 KG-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0065 Cell Name   KG-1
Profile   leukemia, acute myeloblastic Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   59
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute myelogenous leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   Koeffler,H.P. & Golde,D,W.
Registered by   Akiyama,M. Regulation for Distribution   
Comment    Year   1985
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   dilution, twice a week
Cell Number on Passage   Split ratio=1:3 Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   acute myeloblastic leukemia

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0374
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907278
Reference
Pubmed id:6192693Fundamentals of chemotherapy of myeloid leukemia by induction of leukemia cell differentiation.
Hozumi M
Adv Cancer Res. 1983;38():121-69
Pubmed id:6996765Human myeloid leukemia cell lines: a review.
Koeffler HP,Golde DW
Blood. 1980 Sep;56(3):344-50
Pubmed id:6156728Mitogen stimulation of chronic lymphocytic leukemic lymphocytes: defective phytohemagglutinin stimulation independent of immunologic cell surface markers.
Davis S,Rambotti P
Blood. 1980 Aug;56(2):256-61
Pubmed id:306682Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity.
Koeffler HP,Golde DW
Science. 1978 Jun 9;200(4346):1153-4

Images



LOT Information

Cell No.   JCRB0065 Cell Name   
LOT No.   05292000 Lot Specification   distribution
Medium   RPMI1640 with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   3-8 x 10^4 cells/ml Methods for Passages   dilution
Doubling Time   ca 50-60 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   73 Antibiotics Used   free
Passage Number   P20* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium containing 10% DMSO and 20% FCS CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0065 Cell Name   
LOT No.   012599 Lot Specification   distribution
Medium   RPMI1640 + 10% fetal calf serum (ampoule label JCRB9051 is mislabel. Correctly JCRB0065. (Biowhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   dillution
Doubling Time   ca 70hrs Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   84 Antibiotics Used   free
Passage Number   P20* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0065 Cell Name   KG-1
LOT No.   111095 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% heat inactivated fetal bovine serum (ICN Flow 101083) Temperature   37 C
Cell Density at Seeding   1.8x10^5 cells/ml Methods for Passages   Simple dilution to 1-2 x 10^5 cells/ml.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.6x10^6
Viability at cell freezing (%)   78.5 Antibiotics Used   free
Passage Number   P21* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0065 Cell Name   KG-1
LOT No.   091492 Lot Specification   distribution
Medium   RPMI-1640 (070891, 081792) + 10% fetal bovine serum (Flow 007083, Hyclone 1151031) Temperature   37 C
Cell Density at Seeding   1.5x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.6x10^6
Viability at cell freezing (%)   61.4 Antibiotics Used   free
Passage Number   P19* =right. PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0065 Cell Name   KG-1
LOT No.   071692 Lot Specification   distribution
Medium   RPMI1640 (lot.051992/070892) + 10 % FBS (lot.J.R.S.Scientific,DO10400/J.R.H.Bioscience 1B1062) Temperature   37 C
Cell Density at Seeding   1-3x10^5 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA when adherent cells are appeared.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.71x10^6
Viability at cell freezing (%)   84.8 Antibiotics Used   free
Passage Number   P20* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   +
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0065 Cell Name   KG-1
LOT No.   092090 Lot Specification   distribution
Medium   RPMI1640 + 10 % FCS Temperature   37
Cell Density at Seeding   Split ratio=1:3 Methods for Passages   dilution, twice a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P18* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0065 Cell Name   KG-1
LOT No.   112588 Lot Specification   distribution
Medium   RPMI1640 + 10 % FCS Temperature   37
Cell Density at Seeding   Split ratio=1:3 Methods for Passages   dilution, twice a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   77% Antibiotics Used   free
Passage Number   P17* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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