JCRB0078 P-815

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0078 Cell Name   P-815
Profile   mastocytoma, (DBA/2 mouse) Other Name   
Animal   mouse Strain   DBA/2
Genus   Mus Species   musclus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   mastocytoma
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   target cell for cytotoxic T cell assay
Classify   tumor Established by   Dunn-Potter
Registered by   Hasegawa,H. Regulation for Distribution   free
Comment    Year   
Medium   RPMI1640 medium with 10% fetal calf serum. Methods for Passages   Simple dilution.
Cell Number on Passage   split ratio=1/10 Race   
CO2 Conc.   5 % Tissue Sampling   tumor
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2154
Reference
Pubmed id:4616699Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD.
Ralph P,Nakoinz I,Raschke WC
Biochem Biophys Res Commun. 1974 Dec 23;61(4):1268-75
Pubmed id:4208429Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow orgin.
Ralph P,Nakoinz I
Nature. 1974 May 3;249(452):49-51
Pubmed id:4123976Studies on the mechanism of lymphocyte-mediated cytolysis. IV. Specificity of the histamine receptor on effector T cells.
Plaut M,Lichtenstein LM,Gillespie E,Henney CS
J Immunol. 1973 Aug;111(2):389-94

Images



LOT Information

Cell No.   JCRB0078 Cell Name   P-815
LOT No.   062596 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0078 Cell Name   P-815
LOT No.   032696 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum Temperature   
Cell Density at Seeding   Split ratio=1:10 Methods for Passages   Simple dilution
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0078 Cell Name   P-815
LOT No.   052092 Lot Specification   distribution
Medium   RPMI-1640(lot#042992) + FBS 10% (lot#.J.R.Sci., DO10400) Temperature   37 C
Cell Density at Seeding   5-10x10^5 Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.02x10^6
Viability at cell freezing (%)   80.2 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0078 Cell Name   P-815
LOT No.   052791 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37 C
Cell Density at Seeding   dilution 1/10 Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.5x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NP,LD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0078 Cell Name   P-815
LOT No.   060388 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0X10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0078 Cell Name   P-815
LOT No.   061086 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37
Cell Density at Seeding   dilution 1/10 Methods for Passages   Dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   76% Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0078 Cell Name   P-815
LOT No.   11192013 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO cat. # 10091). Temperature   37C
Cell Density at Seeding   1.7 - 3.5 x 10^5 cells/mL Methods for Passages   Simple dilution.
Doubling Time    Cell Number in Vial (cells/1ml)   7.2 x 10^6 (cells/1
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined. Mouse confirmed.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   83.0 Additional information   
Cell No.   JCRB0078 Cell Name   P-815
LOT No.   07212021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   2.0 - 3.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 22 hrs. Cell Number in Vial (cells/1ml)   7.5 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (12 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
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