JCRB0136.2 CHO(pMAM-HSluc)
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0136.2 | Cell Name | CHO(pMAM-HSluc) |
|---|---|---|---|
| Profile | pMAM-HSluc transfected CHO-K1, HSP 70B gene promoter and luciferase reporter gene introduced | Other Name | |
| Animal | hamster, Chinese | Strain | Chinese |
| Genus | Cricetulus | Species | griseus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | ovary | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | pMAM-HSluc transfected CHO-K1 cells. Luciferase activated at 42/43 C for several minutes. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | Character | HSP 70B gene promoter and luciferase gene included | |
| Classify | transformed | Established by | Mizuno,S. and et al. |
| Registered by | Mizuno,S. | Regulation for Distribution | not defined |
| Comment | Refer CHO-K1 cell culture methods. | Year | 1998 |
| Medium | Ham's F12 medium with 10% fetal bovine serum. | Methods for Passages | Cells are treated with 0.25% trypsin and 0.02% EDTA. |
| Cell Number on Passage | split ratio=1/10 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | genetically engineered CHO-K1 cells |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:9538196 | Suppression of thermotolerance development through cycloheximide-induced negative control of stress protein gene expression. Akagawa H,Ishii A,Mizuno S J Biochem. 1998 Feb;123(2):226-32 |
| Pubmed id:9113384 | Stress dose-dependent suppression of heat shock protein gene expression by inhibiting protein synthesis during heat shock treatment. Mizuno S,Ishii A,Murakami Y,Akagawa H Cell Struct Funct. 1997 Feb;22(1):7-13 |
| Pubmed id:8689565 | Heat shock transcription factors: structure and regulation. Wu C Annu Rev Cell Dev Biol. 1995;11():441-69 |
| Pubmed id:8355691 | The DNA-binding activity of the human heat shock transcription factor is regulated in vivo by hsp70. Mosser DD,Duchaine J,Massie B Mol Cell Biol. 1993 Sep;13(9):5427-38 |
| Pubmed id:3422754 | Expression of the firefly luciferase gene in vaccinia virus: a highly sensitive gene marker to follow virus dissemination in tissues of infected animals. Rodriguez JF,Rodriguez D,Rodriguez JR,McGowan EB,Esteban M Proc Natl Acad Sci U S A. 1988 Mar;85(5):1667-71 |
| Pubmed id:3821727 | Firefly luciferase gene: structure and expression in mammalian cells. de Wet JR,Wood KV,DeLuca M,Helinski DR,Subramani S Mol Cell Biol. 1987 Feb;7(2):725-37 |
| Pubmed id:17758108 | Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Ow DW,DE Wet JR,Helinski DR,Howell SH,Wood KV,Deluca M Science. 1986 Nov 14;234(4778):856-9 |
| Pubmed id:3927293 | Isolation and functional analysis of a human 70,000-dalton heat shock protein gene segment. Voellmy R,Ahmed A,Schiller P,Bromley P,Rungger D Proc Natl Acad Sci U S A. 1985 Aug;82(15):4949-53 |
| Pubmed id:5271752 | Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Kao F,Chasin L,Puck TT Proc Natl Acad Sci U S A. 1969 Dec;64(4):1284-91 |
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LOT Information
| Cell No. | JCRB0136.2 | Cell Name | |
|---|---|---|---|
| LOT No. | 100499 | Lot Specification | distribution |
| Medium | Ham's F12 medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x 10^5 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | D.T.=20-24 hrs | Cell Number in Vial (cells/1ml) | 3.8 x 10^6 |
| Viability at cell freezing (%) | 95 | Antibiotics Used | free |
| Passage Number | P8* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as Chinese hamster by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |