JCRB0147 JKT-beta-del

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0147 Cell Name   JKT-beta-del
Profile   The JKT-beta-del cell line isolated from the Jurkat cells{5011} by a cell sorter and lacks the surface expression of the T-cell receptor alpha/beta/CD3 complex. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   T-cell leukemia Metastasis   
Tissue Metastasized    Genetics   Deletion of rearranged T-cell receptor(TCR) C beta gene
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   Jurkat cell clone with a lack of surface expresshion of TCR alpha/beta/CD3 complex {5012, 5010}
Classify   tumor Established by   Kyoizumi,S.
Registered by   Kyoizumi,S. Regulation for Distribution   normal
Comment    Year   1999
Medium   RPMI1640 with 10% heat inactivated fetal calf serum. Methods for Passages   Subculture once a week at split ratio of 1/10.
Cell Number on Passage   split ratio = 1/10 Race   
CO2 Conc.   5 % Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2966
Reference
Pubmed id:10365913Mutant p53: epigenetic mutator of the T-cell receptor via induction of methylation.
Iwamoto KS,Mizuno T,Seyama T,Kyoizumi S
Mol Carcinog. 1999 Jun;25(2):113-21
Pubmed id:1972177Spontaneous loss and alteration of antigen receptor expression in mature CD4+ T cells.
Kyoizumi S,Akiyama M,Hirai Y,Kusunoki Y,Tanabe K,Umeki S
J Exp Med. 1990 Jun 1;171(6):1981-99
Pubmed id:6778951Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.
Gillis S,Watson J
J Exp Med. 1980 Dec 1;152(6):1709-19

Images



LOT Information

Cell No.   JCRB0147 Cell Name   
LOT No.   03082005 Lot Specification   distribution
Medium   RPMI1640 medium with heat-inactivated fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml (1 x 10^5 at log phase) Methods for Passages   dilution
Doubling Time   approx. 28.5 hrs Cell Number in Vial (cells/1ml)   4.3 x 10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   P8 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% inact. FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0147 Cell Name   JKT-beta-del
LOT No.   04272000 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   1.4 x 10^5 cells/sq.cm. Methods for Passages   Cells subcultured by simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH, NP, G6PD(type A). Human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9
D13S317:8,10,11
D7S820:8,10
D16S539:11,12
VWA:18,19
TH01:6,10
AM:X
TPOX:8,10
CSF1PO:11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0147 Cell Name   JKT-beta-del
LOT No.   12132018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3 - 4 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   92.6 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   92.6 Additional information   
Cell No.   JCRB0147 Cell Name   JKT-beta-del
LOT No.   02012021 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.82-2.26x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.99x10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9
D13S317:8,10,11
D7S820:8,10
D16S539:11,12
VWA:18,19
TH01:6,9.3
AM:X
TPOX:8,10
CSF1PO:11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
Images
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