JCRB0239 TCO-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0239 | Cell Name | TCO-1 |
|---|---|---|---|
| Profile | non differentiated cancer | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | thyroid |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | non differenciated cancer |
| Classify | tumor | Established by | Takai,S. |
| Registered by | Takai,S. | Regulation for Distribution | |
| Comment | Year | 1988 | |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum | Methods for Passages | Cells are treated with 0.25 % trypsin. |
| Cell Number on Passage | 5x10^5 cells/10mm dish | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | thyroid gland |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | NT | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2181436 | Hamster cell line suitable for transfection assay of transforming genes. Higashi T,Sasai H,Suzuki F,Miyoshi J,Ohuchi T,Takai S,Mori T,Kakunaga T Proc Natl Acad Sci U S A. 1990 Apr;87(7):2409-13 |
| Images |
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LOT Information
| Cell No. | JCRB0239 | Cell Name | TCO-1 |
|---|---|---|---|
| LOT No. | 11032003 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 4-5 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA. Subculture twice a week. |
| Doubling Time | ca. 26 hrs | Cell Number in Vial (cells/1ml) | 2.9 x 10^6 |
| Viability at cell freezing (%) | 95.1 | Antibiotics Used | free |
| Passage Number | P7* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), AST, LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0239 | Cell Name | |
|---|---|---|---|
| LOT No. | 061989 | Lot Specification | distribution |
| Medium | DMEM + 10 % FCS | Temperature | 37 |
| Cell Density at Seeding | Methods for Passages | 0.25 % trypsin | |
| Doubling Time | Cell Number in Vial (cells/1ml) | ||
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | ||
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |