JCRB0429 KMS-12-BM

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0429 Cell Name   KMS-12-BM
Profile   myeloma, late stage of B-cell differentiation Other Name   KMS-12-BM
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   64
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   myeloma Metastasis   
Tissue Metastasized    Genetics   late stage of B-cell differentiation
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Non tumorigenic, PCA-1(+),CD38(+),OKT9(+)
Classify   transformed Established by   Namba,M. et al.
Registered by   Namba,M. Regulation for Distribution   No commercial purpose only.
Comment    Year   
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Simple dilution.
Cell Number on Passage   5x10^5 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1334
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907281
Reference
Pubmed id:2768132Establishment of five human myeloma cell lines.
Namba M,Ohtsuki T,Mori M,Togawa A,Wada H,Sugihara T,Yawata Y,Kimoto T
In Vitro Cell Dev Biol. 1989 Aug;25(8):723-9

Images



LOT Information

Cell No.   JCRB0429 Cell Name   
LOT No.   02142011 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.4 - 1.9 x 10^5 cells/ml Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   Unknown (16 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0429 Cell Name   
LOT No.   04072005 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   5 - 10 x 10^4 cells/ml Methods for Passages   dilution
Doubling Time   approx. 37 hrs Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   93.3 Antibiotics Used   free
Passage Number   P17 at bank PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0429 Cell Name   
LOT No.   102296 Lot Specification   distribution
Medium   RPMI1640 medium with 10% FCS (heat-inactivation isrecommended). (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   1-5 x 10^5 cells/ml Methods for Passages   dilution JCRB# on tube is mislabeled (correctly, JCRB0429)
Doubling Time    Cell Number in Vial (cells/1ml)   5.3 x 10^5
Viability at cell freezing (%)   87.3 Antibiotics Used   free, (previously treated with MC210, BM1 and BM2)
Passage Number   P13* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0429 Cell Name   KMS-12-BM
LOT No.   08172016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1.9 - 8.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   1.5 - 4 days Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   85.8 Antibiotics Used   free
Passage Number   Unknown (14 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   85.8 Additional information   
Cell No.   JCRB0429 Cell Name   KMS-12-BM
LOT No.   02072023 Lot Specification   distribution
Medium   RPMI1640 medium (GIBCO：Cat No.11875) with 10% heat inactivated fetal bovine serum (SIGMA, Cat No.172012, Lot.12J396) Temperature   37 C
Cell Density at Seeding   2.02-2.66x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.96x10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   p17* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:14
D13S317:8
D7S820:11
D16S539:11
VWA:14,16
TH01:6,9
AM:X
TPOX:8,11
CSF1PO:9
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02- 001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   91 Additional information   
Images
Cell No.   JCRB0429 Cell Name   KMS-12-BM
LOT No.   11022021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.1 - 1.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 2 days. Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
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