JCRB1041 SEKI
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1041 | Cell Name | SEKI |
|---|---|---|---|
| Profile | Human malignant melanoma cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 28 |
| Identity | available | Tissue for Primary Cancer | melanoma |
| Case history | malignant melanoma | Metastasis | No |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Cells are consisted from both melanin containig and melanin non-containing cells. Cells can be cloned by soft agar method. Melanin granules are observed in cells. |
| Classify | tumor | Established by | Shimoyama,M. |
| Registered by | Nose,K. | Regulation for Distribution | |
| Comment | Year | 2002 | |
| Medium | RPMI1640 medium with 20% fetal bovine serum. | Methods for Passages | Dilution. Td = 4-5 days. |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5 % | Tissue Sampling | skin of lower leg |
| Tissue Type | melanoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB1041 | Cell Name | SEKI |
|---|---|---|---|
| LOT No. | 05022003 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 20% fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | Vigorous pipetting and dilution (most of cells are suspension and some cells adhere to substratum). | |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2.1 x 10^6 | |
| Viability at cell freezing (%) | 80.5 | Antibiotics Used | free |
| Passage Number | P18* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), LDH. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | 5% | |
| Viability immediately after thawing (%) | 80.5 | Additional information |
| Cell No. | JCRB1041 | Cell Name | SEKI |
|---|---|---|---|
| LOT No. | 10292020 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO:catalog No.11875) with 20% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) | Temperature | 37 C |
| Cell Density at Seeding | 1.17-1.22x10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.19x10^6 |
| Viability at cell freezing (%) | 93 | Antibiotics Used | free |
| Passage Number | p21* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,11 D13S317:8,9 D7S820:9,12 D16S539:9,10.1,11 VWA:16,18 TH01:9 AM:X TPOX:11 CSF1PO:12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 79.1 | Additional information |
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