JCRB1328 AR-EcoScreen
Cell information
Important Notice(s)On the Distribution of AR-EcoScreen (JCRB1328)
JCRB1328 AR-EcoScreen細胞の分譲について
Cell type：genetically-modified cells (View Pricing Information)
| JCRB No. | JCRB1328 | Cell Name | AR-EcoScreen |
|---|---|---|---|
| Profile | AR-EcoScreen is for evaluation of a rapid in vitro androgen receptor tarnscriptional activation assay. | Other Name | |
| Animal | hamster, Chinese | Strain | Chinese |
| Genus | Cricetulus | Species | griseus |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | ovary |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | This cell is that stably express human androgen receptor, reportor gene (androgen response element linked to luciferase), and firefly luciferase gene. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | |
| Classify | Transfected | Established by | Iida, M. |
| Registered by | Araki, N. | Regulation for Distribution | MTA between applicant and developer is required. |
| Comment | Year | 2008 | |
| Medium | DMEM/F12 medium supplemented with 10% fetal bovine serum, hygromycin (25ug/mL) and zeocin (50 ug/mL). | Methods for Passages | Cells are treated with 0.25% trypsin and 0.02% EDTA. |
| Cell Number on Passage | split ratio=1/10 | Race | |
| CO2 Conc. | 5% | Tissue Sampling | Ovary |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:15950433 | Screening for androgen receptor activities in 253 industrial chemicals by in vitro reporter gene assays using AR-EcoScreen cells. Araki N,Ohno K,Nakai M,Takeyoshi M,Iida M Toxicol In Vitro. 2005 Sep;19(6):831-42 |
| Pubmed id:15713541 | Evaluation of a rapid in vitro androgen receptor transcriptional activation assay using AR-EcoScreen cells. Araki N,Ohno K,Takeyoshi M,Iida M Toxicol In Vitro. 2005 Apr;19(3):335-52 |
| Pubmed id:15110108 | Study on anti-androgenic effects of bisphenol a diglycidyl ether (BADGE), bisphenol F diglycidyl ether (BFDGE) and their derivatives using cells stably transfected with human androgen receptor, AR-EcoScreen. Satoh K,Ohyama K,Aoki N,Iida M,Nagai F Food Chem Toxicol. 2004 Jun;42(6):983-93 |
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LOT Information
| Cell No. | JCRB1328 | Cell Name | AR-EcoScreen |
|---|---|---|---|
| LOT No. | 01302009 | Lot Specification | distribution |
| Medium | DMEM/F12(GIBCO)+hygromycin(25ug/ml)and Zeocin(50ug/ml) with 10% heat inactivated bovine serum(Hyclone GRD0051) | Temperature | 37 C |
| Cell Density at Seeding | 1.8x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.2x10^6 |
| Viability at cell freezing (%) | 98.9 | Antibiotics Used | NT |
| Passage Number | p3* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1328 | Cell Name | AR-EcoScreen |
|---|---|---|---|
| LOT No. | 06122023 | Lot Specification | distribution |
| Medium | DMEM/F12 with 25 ug/mL hygromycin, 50 ug/mL Zeocin, and 10% fetal bovine serum (FBS; GIBCO Cat. # 10437) | Temperature | 37 C |
| Cell Density at Seeding | approx. 1 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 21 hrs | Cell Number in Vial (cells/1ml) | 6.7 x 10^6 |
| Viability at cell freezing (%) | 99 | Antibiotics Used | |
| Passage Number | Unknown (5 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS DMEM/F12 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 98 | Additional information |