JCRB1399 SW620-Luc

Cell information

Important Notice(s)

Leibovitz's L-15 medium

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type：luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1399 Cell Name   SW620-Luc
Profile   Luciferase stably expressing cell line (SW620; Human Colon Cancer). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   51
Identity   available Tissue for Primary Cancer   
Case history   colorectal adenocarcinoma Metastasis   Yes
Tissue Metastasized   colon Genetics   pMSCV-luc transfected SW620 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   puromycin-resistant
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2009
Medium   Leibovitz's L-15 Medium with 10% fetal bovine serum Methods for Passages   Cells are treated with 0.03 % EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio = 1/2 - 1/10 Race   
CO2 Conc.    Tissue Sampling   lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J268
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LOT Information

Cell No.   JCRB1399 Cell Name   SW620-Luc
LOT No.   02232011 Lot Specification   distribution
Medium   Leivobitz's L15(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.2-5.0x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. (Split ratio=1/2-1/20)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.55x10^6
Viability at cell freezing (%)   99.1 Antibiotics Used   NT
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:12
D7S820:8,9
D16S539:9
VWA:16
TH01:8
AM:X
TPOX:11
CSF1PO:13,14
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   0%
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1399 Cell Name   SW620-Luc
LOT No.   07142023 Lot Specification   distribution
Medium   Leibovitz's L-15 medium with 10% fetal bovine serum (FBS ; GIBCO Cat. # 10437, Lot 1072322). Cultivate under normal atmosphere (not under 5% CO2). Temperature   37 C
Cell Density at Seeding   3.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Cultivate under normal atmosphere (not 5% CO2).
Doubling Time   approx. 29 hrs. Cell Number in Vial (cells/1ml)   7.6 x 10^5
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:13
D13S317:12
D7S820:8,9
D16S539:9
VWA:16
TH01:8
AM:X
TPOX:11
CSF1PO:13,14
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - L-15 CO2 Conc.   No
Viability immediately after thawing (%)   91 Additional information   
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