JCRB1509 NCI-H23-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type：luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1509 Cell Name   NCI-H23-Luc
Profile   Luciferase stably expressing cell line (NCI-H23; Human lung adenocarcinoma cell line). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   51-year-old
Identity   available Tissue for Primary Cancer   
Case history   adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   pMSCV-luc transfected. Luciferase stably expressing cell line. Oncogene: myc +; src +; abl +; erb +; ras +; sis -
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   puromycin-resistant
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2011
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio = 1/3 - 1/6 Race   Black
CO2 Conc.   5% Tissue Sampling   lung
Tissue Type   lung

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W97
Images



LOT Information

Cell No.   JCRB1509 Cell Name   NCI-H23-Luc
LOT No.   01252013 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine (Sigma Cat#F0926, lot#027K03911) Temperature   37 C
Cell Density at Seeding   0.6-5.2x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.5x10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   p16* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:12
D7S820:9,10
D16S539:11
VWA:16,17
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1509 Cell Name   NCI-H23-Luc
LOT No.   01192024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; NICHIREI Cat. # 174112, Lot 20M00k) Temperature   37 C
Cell Density at Seeding   0.7 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (19 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:12
D7S820:9,10
D16S539:11
VWA:16,17
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   
CLOSE