JCRB1592 Hep G2-Luc

Cell information

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発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

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Information for introduced genes, etc in luciferase expressing cell lines

Cell type：luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1592 Cell Name   Hep G2-Luc
Profile   Luciferase stably expressing cell line (Hep G2: Human Hepatoma Cell Line). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   15-year-old
Identity   available Tissue for Primary Cancer   liver
Case history   liver cancer Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected Hep G2 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Hepatoma cell line. alpha-fetoprotein (+), HLA-A 02/2402. Puromycin-resistant.
Classify   tumor Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2014
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   Cells harvested after treatment with 0.25% trypsin.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   differentiated liver carcinoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG47
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LOT Information

Cell No.   JCRB1592 Cell Name   Hep G2-Luc
LOT No.   06302014 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.25-3.39x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.04x10^6
Viability at cell freezing (%)   95.7 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,13
D7S820:10
D16S539:12,13
VWA:17
TH01:9
AM:X,Y
TPOX:8,9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1592 Cell Name   Hep G2-Luc
LOT No.   03102023 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum (Biowest cat.No. S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   5.56-5.76x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin (GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.80x10^6
Viability at cell freezing (%)   97.7 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,13
D7S820:10
D16S539:12,13
VWA:17
TH01:9
AM:X,Y
TPOX:8,9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   91.7 Additional information   
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