JCRB1853 DR-EcoScreen-HS

Cell information

Important Notice(s)

JCRB1853 DR-EcoScreen-HS細胞の分譲について

On the Distribution of DR-EcoScreen-HS (JCRB1853)

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1853 Cell Name   DR-EcoScreen-HS
Profile   A cell line for evaluation of a rapid in vitro aryl hydrocarbon receptor transcriptional activation assay. Higher responsiveness to TCDD than DR-EcoScreen. Other Name   
Animal   mouse Strain   C57L
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   This cell line was stably transfected with a reportor plasmid, pIND-DCDR7 (dioxin-responsive element linked to luciferase).
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Hygromycin-resistant.
Classify   transformed Established by   Iida, M.
Registered by   Iida, M. Regulation for Distribution   MTA between applicant and developer is required.
Comment    Year   2019
Medium   Alpha-MEM with 5% FBS. 0.15mg/ml hygromycin is recommended to be added during cultivation and maintenance. (Hygromycin should be removed at the assay of test samples.) Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA. Passage twice or three times/week.
Cell Number on Passage   split ratio 1/10 Race   
CO2 Conc.   5% Tissue Sampling   Liver (Hepa1c1c7 cell derivative)
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_B3MB
Reference
Pubmed id:18835618In vitro screening for aryl hydrocarbon receptor agonistic activity in 200 pesticides using a highly sensitive reporter cell line, DR-EcoScreen cells, and in vivo mouse liver cytochrome P450-1A induction by propanil, diuron and linuron.
Takeuchi S,Iida M,Yabushita H,Matsuda T,Kojima H
Chemosphere. 2008 Dec;74(1):155-65

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LOT Information

Cell No.   JCRB1853 Cell Name   DR-EcoScreen-HS
LOT No.   03262021 Lot Specification   distribution
Medium   MEM alpha (GIBCO,Cat.No.12571-063) with 5% heat inactivated fetal bovine serum(SIGMA,Cat#172012-500ML;Lot#12J396) and 0.15 mg/ml hygromycin (INVIVOGEN #ant-hg-1) Temperature   37 C
Cell Density at Seeding   3.15-3.92x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.01x10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
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