JCRB1980 MDCK-EKARrEV-NLS-dEREG Cell information
Cell type：genetically-modified cells
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JCRB No.
JCRB1980
Cell Name
MDCK-EKARrEV-NLS-dEREG
Profile
epiregulin (EREG)-deficient MDCK cell line expressing FRET biosensor EKARrEV-NLS.
Other Name
Animal
dog
Strain
Genus
Canis
Species
familiaris
Sex
F
Age
Identity
not done
Tissue for Primary Cancer
Case history
normal
Metastasis
Tissue Metastasized
Genetics
mutation of EREG and FRET biosensor EKARrEV-NLS expressing
Life Span
infinite
Crisis PDL
Morphology
epithelial-like
Character
Classify
transformed
Established by
Shuhao Lin
Registered by
Michiyuki MATSUDA
Regulation for Distribution
Comment
Year
Medium
DMEM with 10% FBS
Methods for Passages
0.25% trypsin + 0.02% EDTA
Cell Number on Passage
Race
CO2 Conc.
5%
Tissue Sampling
kidney
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Reference
Pubmed id:34667080 Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration.
Movies
LOT Information
Cell No.
JCRB1980
Cell Name
MDCK-EKARrEV-NLS-dEREG
LOT No.
10242023
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium (GIBCO cat No. 11885) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396).
Temperature
37 C
Cell Density at Seeding
9.09x10^3 cells/sq.cm
Methods for Passages
Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time
NT
Cell Number in Vial (cells/1ml)
2.86x10^6
Viability at cell freezing (%)
99
Antibiotics Used
free
Passage Number
p2*
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Exoteric Gene
NT
Medium for Freezing
BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD)
CO2 Conc.
5%
Viability immediately after thawing (%)
96
Additional information
