JCRB1993 MDCK-WT-EKARrEV-NLS-LDR

Cell information

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1993 Cell Name   MDCK-WT-EKARrEV-NLS-LDR
Profile   MDCK cell line expressing FRET biosensor EKARrEV-NLS and Lyn-targeted FRB (LDR). Other Name   
Animal   dog Strain   
Genus   Canis Species   familiaris
Sex   F Age   
Identity   not done Tissue for Primary Cancer   
Case history   normal Metastasis   
Tissue Metastasized    Genetics   FRET biosensor EKARrEV-NLS and Lyn-targeted FRB (LDR) expressing.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Shuhao Lin
Registered by   Michiyuki MATSUDA Regulation for Distribution   
Comment    Year   
Medium   DMEM with 10% FBS Methods for Passages   0.25% trypsin + 0.02% EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Reference
Pubmed id:34667080Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration.
Lin S,Hirayama D,Maryu G,Matsuda K,Hino N,Deguchi E,Aoki K,Iwamoto R,Terai K,Matsuda M
Life Sci Alliance. 2022 Jan;5(1):

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LOT Information

Cell No.   JCRB1993 Cell Name   MDCK-WT-EKARrEV-NLS-LDR
LOT No.   12132023 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (GIBCO:catalog No.11885-084) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396). Temperature   37 C
Cell Density at Seeding   1.60-1.71x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.03x10^6
Viability at cell freezing (%)   99.56 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   97.26 Additional information   Vials are mislabeled as MDCK-WT-EKARrEV-NSL-LDR. Correct name is MDCK-WT-EKARrEV-NLS-LDR.
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