-Detailed Information [JCRB9060]-

JCRB9060 MRC-9

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB9060	Cell Name	MRC-9
Profile	fetal lung, normal diploid fibroblast	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex		Age
Identity	available	Tissue for Primary Cancer	lung
Case history		Metastasis
Tissue Metastasized		Genetics	fetal lung, diploid female
Life Span		Crisis PDL
Morphology		Character	fetal lung, diploid female
Classify		Established by	Jacobs,J.P.
Registered by		Regulation for Distribution	Following rules from ATCC
Comment	Prepared from CCL 212. Population doubling is 28 at P4.	Year	1987
Medium	Eagle's minimal essential medium with non essential amino acids and 10% fetal calf serum.	Methods for Passages	Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage	1/2 - 1/6	Race
CO2 Conc.	5 %	Tissue Sampling	lung
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_2629

Reference
Pubmed id:113411	Characteristics of a serially propagated human diploid cell designated MRC-9. Jacobs JP,Garrett AJ,Merton R J Biol Stand. 1979 Apr;7(2):113-22

Images

LOT Information

061395
F00990
022089

Cell No.	JCRB9060	Cell Name	MRC-9
LOT No.	061395	Lot Specification	distribution
Medium	Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum	Temperature	37 C
Cell Density at Seeding	9.8x10^5 cells/100mm dish	Methods for Passages	Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1.25x10^6
Viability at cell freezing (%)	60.0	Antibiotics Used	free
Passage Number	P7	PDL	34PDL
Sterility: MYCOPLASMA	NT	Sterility: BACTERIA	NT
Sterility: FUNGI	NT	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12,13 D13S317:8,11 D7S820:10,11 D16S539:12 VWA:16,17 TH01:6,8 AM:X TPOX:11 CSF1PO:12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB9060	Cell Name	MRC-9
LOT No.	F00990	Lot Specification	distribution
Medium	Eagle's MEM with NEAA and 10% FCS (BioWhittaker lot 3M0581)	Temperature	37
Cell Density at Seeding	1/2-1/6	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	8.0 x 10^5
Viability at cell freezing (%)	90.0	Antibiotics Used	free
Passage Number	P7	PDL	ca 33
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	confirmed as human by isoenzymology (NP,G6PD,AST)
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information	Lot# is labelled as R925F00990.

Cell No.	JCRB9060	Cell Name	MRC-9
LOT No.	022089	Lot Specification	distribution
Medium	MEM w/ non essential amino acids + FCS 10%	Temperature	37
Cell Density at Seeding	1/2 - 1/6	Methods for Passages	0.02% EDTA and 0.25% trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.4x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P7	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information