JCRB3031 AP38S
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB3031 | Cell Name | AP38S |
|---|---|---|---|
| Profile | Hereditary disease with high cancer risk Fanconi anemia (autosomal recessive) chromosomal breakage syndrome high sensitivity to mitomycin C | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 9-year-old |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Fanconi anemia (A) | Metastasis | |
| Tissue Metastasized | Genetics | MMC sensitive(lymphocyte), FANCA mutation, karyotype:46,XX,inv(5) | |
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | skin fibroblast |
| Classify | primary | Established by | Sasaki M. |
| Registered by | Radiation Biology Center, Kyoto University | Regulation for Distribution | |
| Comment | identical twin of AP38P | Year | |
| Medium | Methods for Passages | ||
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5% | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
LOT Information
| Cell No. | JCRB3031 | Cell Name | AP38S |
|---|---|---|---|
| LOT No. | 10142015 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA12J396,non HI) | Temperature | 37 C |
| Cell Density at Seeding | 1.0-5.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/3-1/4) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.38x10^6 |
| Viability at cell freezing (%) | 98.2 | Antibiotics Used | free |
| Passage Number | p6 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,11 D13S317:9 D7S820:10,11 D16S539:11,14 VWA:15,17 TH01:6,9 AM:X TPOX:8,11 CSF1PO:12,15 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | NT | Additional information |