PCR Primers for Amplifying STR Markers
Following data was taken from the National Institute of Standards and Technology (NIST) and modified a bit. Please refer original information and STR DNA Internet DataBase in the NIST.

STR Locus

Chromosomal Position

GenBank Accession

Primer Sequence (5'-->3')

References
see below

ACTBP2 (SE33)

6

V00481

(HSAC04)

5'-AATCTGGGCGACAAGAGTGA-3'

5'-ACATCTCCCCTACCGCTATA-3'

6

Amelogenin

Xp22.1-.3 Yp11.2

M55418

M55419

5'-ACCTCATCCTGGGCACCCTGG-3'

5'-AGGCTTGAGGCCAACCATCAG-3'

96

APOA2

11q23-qter

J00048

5'-GTGACAGAGGGAGACTCCATTAAA-3'

5'-GGAGCAGTCCTAGGGCCGCGCCGT-3'

6

APOB

2p24-p23

J04838

5'-ACATTGATTCCTTGGAGTTTCTCTA-3'

5'-GGGCAAAAGAGCGAGACTCCATCTC-3'

6

ARA

Xcen-q13

M21748

5'-TCCAGAATCTGTTCCAGAGCGTGC-3'

5'-GCTGTGAAGGTTGCTGTTCCTCAT-3'

2,3

CD4

12p12-pter

M86525

5'-TTGGAGTCGCAAGCTGAACTAGCG-3'

5'-CCAGGAAGTTGAGGCTGCAGTGAA-3'

3

CRYGBC

2q33-q35

M19364

5'-CCGACAGAGTGAGACTCCATCTCA-3'

5'-ACAGGATCCTATCTTCTCAGGAGG-3'

6

CSF1PO

5q33.3-q34

X14720

5'-AACCTGAGTCTGCCAAGGACTAGC-3'

5'-TTCCACACACCACTGGCCATCTTC-3'

3

CYAR04 (P450)

15q21.1

M30798

5'-GGTAAGCAGGTACTTAGTTAGCTAC-3'

5'-GTTACAGTGAGCCAAGGTCGTGAG-3'

3

D21S11

21q11-q21

M84567

5'-GTGAGTCAATTCCCCAAG-3'

5'-GTTGTATTAGTCAATGTTCTCC-3'

2,6

D6S366

6q21-qter

L25870

5'-AGAGGTTACAGTGAGCCGAGATTG-3'

5'-GAAGTCCTAACAGAATGGAAGGTCC-3'

3

D7S460

7

  

5'-AATACCCCAAGGGGTGGTAA-3'

5'-CATTGATGAACAGTTCAAGCA-3'

6

DYS19

Y

X77751

5'-CTACTGAGTTTCTGTTATAGT-3'

5'-ATGGCATGTAGTGAGGACA-3'

95

F13A1

6p24-25

M21986

5'-GAGGTTGCACTCGAGCCTTTGCAA-3'

5'-TTCCTGAATCATCCCAGAGCCACA-3'

3

F13B

1q31-q32.1

M64554

5'-TGAGGTGGTGTACTACCATA-3'

5'-GATCATGCCATTGCACTCTA-3'

149

FABP

4q28-q31

M18079

5'-GTAGTATCAGTTTCATAGGGTCACC-3'

5'-CAGTTCGTTTCCATTGTCTGTCCG-3'

3,6

FES/FPS

15q25-qter

X06292

5'-GCTTGTTAATTCATGTAGGGAAGGC-3'

5'-GTAGTCCCAGCTACTTGGCTACTC-3'

3

FIBRA (FGA)

4q28

M64982

5'-ATTATCCAAAAGTCAAATGCCCCATAGG-3'

5'-ATCGAAAATATGGTTATTGAAGTAGCTG-3'

6

HPRTB

Xq26

M26434

5'-ATGCCACAGATAATACACATCCCC-3'

5'-CTCTCCAGAATAGTTAGATGTAGG-3'

2,3

LIPOL (LPL)

8p22

M15856

5'-CTGACCAAGGATAGTGGGATATAG-3'

5'-GGTAACTGAGCGAGACTGTGTCT-3'

3

MBP

18q23-pter

M63599

5'-GGACCTCGTGAATTACAATC-3'

5'-ATTTACCTACCTGTTCATCC-3'

89

PLA2A1

12q23-qter

M22970

5'-GGTTGTAAGCTCCATGAGGTTAGA-3'

5'-TTGAGCACTTACTATGTGCCAGGCT-3'

3

RENA4

1q32

M10151

5'-AGAGTACCTTCCCTCCTCTACTCA-3'

5'-CTCTATGGAGCTGGTAGAACCTGA-3'

3

TFIIDA

  

M34960

(mRNA)

5'-CAATGATGCCTTATGGCACTGGAC-3'

5'-TGTGAGAGTCTGTGAGTGGAAGAG-3'

6

TH01

11p15-15.5

D00269

5'-GTGGGCTGAAAAGCTCCCGATTAT-3'

5'-ATTCAAAGGGTATCTGGGCTCTGG-3'

2,3

TPOX

2p23-2pter

M68651

5'-ACTGGCACAGAACAGGCACTTAGG-3'

5'-GGAGGAACTGGGAACCACACAGGT-3'

153

VWA

12p12-pter

M25858

5'-CCCTAGTGGATGATAAGAATAATC-3'

5'-GGACAGATGATAAATACATAGGATGGATGG-3'

4


References
2. Fregeau, C.J. and Fourney, R.M. (1993) DNA typing with fluorescently tagged short tandem repeats: a sensitive and accurate approach to human identification. BioTechniques 15: 100-119.
3. Hammond, H.A., Jin, L., Zhong, Y., Caskey, C.T. and Chakraborty, R. (1994) Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am. J. Hum. Genet. 55: 175-189.
4. Kimpton, C.P., Gill, P., Walton, A., Urquhart, A., Millican, E.S. and Adams, M. (1993) Automated DNA profiling employing multiplex amplification of short tandem repeat loci. PCR Meth. Appl. 3: 13-22.
6. Sprecher, C.J., Puers, C., Lins, A.M. and Schumm, J.W. (1996) General approach to analysis of polymorphic short tandem repeat loci. BioTechniques 20: 266-276.
89. Lorente, M., Lorente, J.A., Wilson, M.R., Budowle, B. and Villanueva, E. (1993) Composite PAGE: an alternative method for increased separation of amplified short tandem repeat alleles. Int. J. Leg. Med. 106: 69-73.
95. Roewer, L. and Epplen, J.T. (1992) Rapid and sensitive typing of forensic stains by PCR amplification of polymorphic simple repeat sequences in case work. Forensic Sci. Int. 53: 163-171.
96. Sullivan, K.M., Mannucci, A., Kimpton, C.P. and Gill, P. (1993) A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X-Y homologous gene amelogenin. BioTechniques 15: 637-641.
149. Nishimura, D.Y. and Murray, J.C. (1992) A tetranucleotide repeat for the F13B locus. Nucleic Acids Res. 20: 1167
153. Huang, N.E., Schumm, J.W. and Budowle, B. (1995) Chinese population data on three tetrameric short tandem repeat loci--HUMTH01, TPOX, and CSF1PO--derived using multiplex PCR and manual typing. Forensic Sci. Int. 71: 131-136.