| Date accepted: | 11/05/85 |
| Animal: | bovine |
| Scientific Name: | Bos taurus |
| Tissue: | carotid artery |
| Medium: | Eagle's minimal essential medium with 10% fetal calf serum |
| Passage method: | Cells are treated with 0.02 % EDTA and 0.05 % trypsin. |
| Cell no. at passage: | 8x10^5 cells/100mm dish |
| Case history: | normal |
| Genetics: | carotid artery endothelium, primary |
| Life span: | finite |
| Crisis PDL: | 60-70 pas. |
| Morphology: | epithelial-like |
| Characteristics: | carotid artery endothelium, primary |
| Classification: | normal |
| Established by | Hagiwara,H. & Inada,Y. |
| Deposited by | Inada,Y. |
| Cell bank maintaining these cells. | NIHS(JCRB) |
| References: | (1) |
| Comments: | Do not culture over passage 25. |
| Cell culture, Isozyme analysis, Others(1), |
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No extradocument. |
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[ Lot No.111685: token/seed(JCRB0099) ] |
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Date cell culture started:11/16/85 Concentration of cells in an ampule: 10^6 cell/ml Culture medium: MEM + FCS 10% Passage method: 0.02% EDTA and 0.05% trypsin. Cell No. at passage: 8x10^5 cells/10cm dish Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, AST,G6PD,LD,MD,MPI,NP,PEPB (bovine confirmed) showed original animals derived. Anchorage dependency: Yes Memo: Caution! ampule no marked (Date downloaded: 1999-06-02) |
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[ Lot No.022586: distribution(JCRB0099) ] |
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Date cell culture started:02/25/86 Concentration of cells in an ampule: 1.7x10^6 cell/ml Culture medium: MEM + FCS 10% Passage method: 0.02% EDTA and 0.05% trypsin. Cell No. at passage: 8x10^5 cells/10cm dish Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, AST,G6PD,LD,MD,MPI,NP,PEPB showed original animals derived. Anchorage dependency: Yes Tumorgenesity: Not tested (Date downloaded: 1999-06-02) |
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[ Lot No.070488: distribution(JCRB0099) ] |
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Date cell culture started:07/04/88 Concentration of cells in an ampule: 2.0x10^6 cell/ml Culture medium: MEM + FCS 10% Passage method: 0.02% EDTA and 0.05% trypsin. Cell No. at passage: 8x10^5 cells/10cm dish Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. (Date downloaded: 1999-06-02) |
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[ Lot No.022693: distribution(JCRB0099) ] |
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Date cell culture started:01/13/93 Concentration of cells in an ampule: 2.1x10^6 cell/ml Culture medium: Eagle's minimal essential medium with Heparin-Na(100 ug/ml) and 10% FBS (endothelial growth suppl.) Passage method: Treat twice with 0.25% trypsin-0.02% EDTA. 7% CO2 is recommended. Collagen or gelatin coated dish. Cell No. at passage: 8x10^5 cells/10cm dish Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Anchorage dependency: Yes (Date downloaded: 1999-06-02) |
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[ Lot No.121495: distribution(JCRB0099) ] |
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Date cell culture started:11/28/95 Concentration of cells in an ampule: 2.9x10^6 cell/ml Culture medium: Eagle's minimal essential medium with 10% fetal bovine serum (Cell Culture Lab.00939-1) Freezing medium: Culture medium with 5% DMSO. Passage method: Wash cells with PBS(-) and treated with 0.25% trypsin and 0.02% EDTA for 5 min. at 37 C. Cell No. at passage: 1.3 x 10^5 cells/ml Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, LDH, G6PD, NP bovine confirmed showed original animals derived. Anchorage dependency: Yes Memo: from lot.111685 by Nishimura,K. (Date downloaded: 1999-06-02) |
