| Date accepted: | 07/10/2002 |
| Previous cell Number: | NEW |
| Animal: | human |
| Sex and Age: | M :43-year-old |
| Race: | Japanese |
| Scientific Name: |
Homo sapiens |
| Case history: | gallbladder carcinoma |
| Classification: | tumor |
| Tissue prepared: | malignant peritoneal effusion |
| Origin of tumor: | malignant peritoneal effusion |
| Metastatic tissue: | peritoneal effusion |
| History of the cell: | Hirakawa,H. -> HSRRB -> JCRB |
| Medium: | Dulbecco's modified Eagle's medium 0.5mM pyruvate, 2mM glutamine and 10% fetal calf serum. |
| CO2 Concentration: | 5 % |
| Genetics: | Chromosome number distribute in a broad range from 52 to 139. |
| Life span: | infinite |
| Morphology: | clusters with monolayer cells |
| Characteristics: | Only TA-4 antigen detected in the spent medium while high levele SLX, CA19-9, SPan-1 and TA-4 were observed in the serum of patient. |
| Cell ID data: | available |
| DNA Profile (STR): | D5S818:10,12
D13S317:8,11
D7S820:10,11
D16S539:9,13
vWA:15,16
TH01:7,10
Amelogenin:X
TPOX:8,11
CSF1PO:10,12
|
| Virus DNA detected: | CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested] -- Description |
| Established by | Yamada,N. et.al. |
| Deposited by | Hirakawa,H. |
| Cell bank: | HSRB(JCRB) |
| References: | (1) |
| Comments: | Cells are tumorigenic in nude mice. Poorly defferentiated adenocarcinoma. The cell line was identified to be a unique cell line by STR-PCR experiment (2002.June). |
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