JCRB0228:A-172

JCRB0228 [A-172]

Date accepted:	08/27/1987
Animal:	human
Scientific Name:	Homo sapiens
Tissue prepared:	brain
Medium:	Dulbecco's modified Eagle's medium with 10% fetal calf serum.
Passage method:	Cells are treated with 0.25 % trypsin.
CO₂ Concentration:	5 %
Cell no. at passage:	2x10^4 cells/ml
Genetics:	glioblastoma
Characteristics:	glioblastoma
Cell ID data:	available
DNA Profile (STR):	D5S818:11,12 D13S317:11 D7S820:11 D16S539:12 vWA:20 TH01:6,10 Amelogenin:XY TPOX:8,11 CSF1PO:9,12
Virus DNA detected:	CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive, nt/not tested] -- Description
Established by	Giard,D.J. et al.
Deposited by	Egashira,Y.
Cell bank:	FDSC(JCRB)
References:	(1)

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Mycoplasma test, Isozyme analysis, STR-PCR(1), STR-PCR(2), Freezing process,

[ Extra document(s) ]

Additional Information(1)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 030796: token/seed (JCRB0228) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

100
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle's medium with 10% fetal bovine serum (JRH3M2215).

Freezing medium:

Freezing medium with 5% DMSO.

Passage method:

Cells are treated with 0.25% trypsin.

Growth temperature:

Cell No. at passage:

6.87x10^5 cells/ml

Memo:

[ Additional Data ]

Mycoplasma test,

STR-PCR(1),

STR-PCR(2),

Freezing process,

(Date downloaded: 2009-03-16)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Giard,D.J., Aaronson,S.A., Todaro,G.J., Arnstein,P., Kersey,J.H., Dosik,H., and Parks,W.P. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors, J. Natl. Cancer Inst., 51: 1417-1423, 1973.