JCRB0260:SAS

JCRB0260 [SAS]

Date accepted:	03/13/1989
Animal:	human
Scientific Name:	Homo sapiens
Tissue prepared:	tongue
Medium:	45% Dulbecco's modified Eagle's medium with 45% Ham's F12 medium and 10% fetal calf serum
Passage method:	Cells are treated with 0.02% EDTA and 0.1% trypsin.
CO₂ Concentration:	5 %
Cell no. at passage:	10^5 cells/60mm dish
Genetics:	tongue, squamous carcinoma
Characteristics:	tongue, squamous carcinoma
Cell ID data:	available
DNA Profile (STR):	D5S818:9 D13S317:10,12 D7S820:11,12 D16S539:12,13 VWA:10,14,17 TH01:6,7 Amelogenin:X TPOX:11,12 CSF1PO:11
Virus DNA detected:	CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(nt) [-/negative, +/positive, nt/not tested] -- Description
Established by	Takahashi,K.
Deposited by	Sato, K.
Special notice:	Contact to K. Sato at distribution of cells.
Cell bank:	FDSC(JCRB)
References:	(1,2)
Comments:	Derived from primary focus.

(* The format of the table was revised on Apr.25.2003.)

[ Picture(s) ]

Cells(1), Cells(2), Cells(3), Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, STR-PCR(1), STR-PCR(2), STR-PCR(3), Freezing process, Others(1), Others(2), Others(5),

[ Extra document(s) ]

Additional Information(1)

Date downloaded:

[ Record(s) of stored ampules ]

[ Lot No.: 031489: token/seed (JCRB0260) ]

Passage Number,

Date cell culture started:

Concentration of cells in an ampule:

Antibiotics used :

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

LD,G6PD,NP,PepB,ASTMD,MPI.

[ Culture condition of this lot.]

Culture medium:

DMEM + Ham's F12 (1:1) + FCS 10%

Passage method:

0.02% EDTA and 0.1% trypsin.

Growth temperature:

Cell No. at passage:

Additional comments:

Viability was 97% prior to freezing.

[ Additional Data ]

(Date downloaded: 2008-07-09)

[ Lot No.: 052695: FDSC/distribution (JCRB0260) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

98.6
Antibiotics used :free

Tests for bacteria and fungi were netative, but mycoplasma test has not completed.

Anchorage dependency:

[ Culture condition of this lot.]

Culture medium:

Dulbecco's modified Eagle medium 50% with Ham's F12 medium 50% and 10% fetal bovine serum (JRH12103)

Passage method:

Treat cells with 0.25% trypsin and 0.02%.

Growth temperature:

Cell No. at passage:

6.44x10^4 cells/ml

Memo:

[ Additional Data ]

(Date downloaded: 2008-07-09)

[ Lot No.: 120798: seed (JCRB0260) ]

Cells are propagated from the stock,

Passage Number,

Cell culture was started without cloning from the previous stock.

Date cell culture started:

Concentration of cells in an ampule:

Viability under microscope (%):

90.6
Antibiotics used :free

Tests for mycoplasma, bacteria, and fungi were negative.

Tested Isoenzymes

G6PD, LD and NP were analyzed..

Anchorage dependency:

Cell Identification:

[ Culture condition of this lot.]

Culture medium:

45% Dulbecco's modified Eagle's medium with 45% Ham's F12 medium and 10% fetal bovine serum.

Freezing medium:

Culture medium with 5% DMSO.

Passage method:

Cells harvested after treatment with 0.01% trypsin and 0.02% EDTA.

Growth temperature:

CO₂ concentration:

Cell No. at passage:

2.2x10^4 cells/sq.cm.

Memo:

[ Additional Data ]

Mycoplasma test,

Mycoplasma (PCR),

Isozyme analysis,

STR-PCR(1),

STR-PCR(2),

STR-PCR(3),

Freezing process,

(Date downloaded: 2008-07-09)

Exported from cellroot.dbf and cellspec.dbf.

References

1. Takahashi,K., Kanazawa,H., Akiyama,Y., Tazaki,S., Takahara,M., Muto,T., Tanzawa,H., and Sato,K. Establishment and characterization of a cell line (SAS) from poorly defferentiated human squamous cell carcinoma of the tongue, J. Jpn. Stomatol. Soc., 38(1): 20-28, 1989.

2. Endo,H., Noda,H., Kinoshita,N., Inui,N., and Nishi,Y. Formation of a transplacental mutagen, 1,3-Di(4- sulfamoylphenyl)triazene, from sodium nitrite and sulfanilamide in human gastric juice and in the stomachs of hamsters, J. Natl. Cancer Inst., 65: 547-551, 1980.