| Date accepted: | 07/14/88 |
| Animal: | mouse |
| Scientific Name: | Mus musclus |
| Tissue: | hybrid |
| Medium: | Dulbecco's modified Eagle's medium with 10% fetal calf serum and 800 ug/ml G418. |
| Passage method: | Cells are treated with 0.25% trypsin and 0.02% EDTA for 3 min. at 37 C. |
| Cell no. at passage: | 1-2x10^4 cells/sq.cm. |
| Genetics: | The cell line contains human chromosome #7 |
| Life span: | infinite |
| Characteristics: | contains human chromosome #7 |
| Classification: | hybrid cell |
| Established by | Oshimura,M. |
| Deposited by | Oshimura,M. |
| Cell bank maintaining these cells. | YCBR(JCRB) |
| References: | (1) |
| Cell culture, Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, Freezing process, Others(1), |
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No extradocument. |
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[ Lot No.:031790: test (JCRB0726) ] |
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Date cell culture started:03/18/90 Concentration of cells in an ampule: 1.1x10^7 cell/ml Culture medium: DMEM + 10% FCS + 800 ug/ml G418 Growth temperature: 37 Passage method: trypsin Cell No. at passage: split 1:4 Antibiotics used :G418 [Pictures]
(Date downloaded: 1999-08-11) |
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[ Lot No.:051595: FDSC/seed (JCRB0726) ] |
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Concentration of cells in an ampule: 2.0x10^6 cell/ml Culture medium: Dulbecco's modified Eagle's medium with 10% fetal calf serum and 800 ug/ml G418. Growth temperature: 37 C Passage method: cells are treated with trypsin Cell No. at passage: split ratio=1:4 Plating efficiency: 70% Antibiotics used :free Tests for mycoplasma, bacteria, and fungi were negative. Anchorage dependency: Yes Memo: Adjust medium pH to 7.1 by 100mM HEPES. [Pictures]
(Date downloaded: 1999-08-11) |
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[ Lot No.:090196: seed (JCRB0726) ] |
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Date cell culture started:08/21/96 Concentration of cells in an ampule: 1.97x10^6 cell/ml Culture medium: Dulbecco's modified Eagle's medium with 800ug/ml G-418 and 10% fetal bovine serum (JRH Biosciences) Growth temperature: 37 C Freezing medium: Culture medium with 5% DMSO. Passage method: Harvest cells with 0.25% trypsin and 0.02% EDTA at 37 C for 3 min. Cell No. at passage: 1.3-2x10^4 cells/sq.cm. Antibiotics used :free except G-418 as a selection of the chromosome Tests for mycoplasma, bacteria, and fungi were negative. Tested Isoenzymes, G6PD, LDH, NP. (mouse) showed original animals derived. Chromosome frequency distribution: Karyotype under examination. Anchorage dependency: Yes Exogenous gene(s): human chromosome Memo: From new lot from Kihara Inst.Biol.Res.(Tanabe,H) [Pictures] Cell culture, Mycoplasma test, Mycoplasma (PCR), Isozyme analysis, Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, Chromosome, Freezing process, Others(1), (Date downloaded: 1999-08-11) |
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[ Lot No.:090296: distribution (JCRB0726) ] |
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Date cell culture started:08/21/96 Tests for mycoplasma, bacteria, and fungi were negative. Chromosome frequency distribution: Under examination. Memo: See lot.090196 for detailes. [Pictures] Cell culture, Mycoplasma test, Mycoplasma (PCR), Freezing process, (Date downloaded: 1999-08-11) |
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[ Lot No.:000000: deposit (JCRB0726) ] |
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[Pictures]
(Date downloaded: 1999-08-11) |
(Abstract)
